摘要
目的探讨嗜麦芽窄食单胞菌耐药株是否存在主动外排作用,以揭示嗜麦芽窄食单胞菌对氟喹诺酮类药物(FQS)的耐药机制。方法应用琼脂稀释法检测嗜麦芽窄食单胞菌耐药株和敏感株在加入主动外排泵抑制剂羰基氰氯苯腙(CCCP)后对环丙沙星、左氧氟沙星的最低抑菌浓度(MIC)变化情况。结果55株嗜麦芽窄食单胞菌对FQS耐药率较低,CCCP在体外能增强FQS抗菌活性,主动外排机制既存在于FQS耐药株,也存在于FQS敏感株,对耐药株的影响更大。结论嗜麦芽窄食单胞菌对FQS耐药与主动外排泵有关,泵抑制剂可部分降低这种耐药性。
Objective To explore the drug resistance mechanism of Stenotrophomonas maltophilia on fluoroquinolones（FQS） ,and to investigate whether the active efflux system exists in them. Methods The influence of active efflux inhibitors carbonyl cyanide m-chlorophenylhydrazone （CCCP） on minimal inhibitory concentration （MIC） of Stenotrophomonas maltophilia to ciprofloxacin and levofloxacin were measured by agar dilution method. Results Of 55 strains of Stenotrophomonas maltophilia, the resistant ratio of FQS was lower. In vitro CCCP enhanced the activities of FQS. Efflux mechanism existed not only in FQS resistant strains, but also in FQS sensitive strains. But the effect of CCCP on FQS resistant strains was much stronger than that of FQS susceptible strains. Conclusion FQS resistance in Stenotrophomonas maltophilia is related to active efflux pump. Efflux pump inhibitors may partly reduce the resistance.
出处
《检验医学与临床》
CAS
2008年第7期402-403,共2页
Laboratory Medicine and Clinic
关键词
嗜麦芽窄食单胞菌
左氧氟沙星
环丙沙星
抗药性
细菌
外排泵
stenotrophomonas maltophilia
levofloxacin
ciprofloxacin, drug resistance, bacterial
efflux pump
