摘要
目的:观察新西兰大白兔腰椎关节突关节破坏致椎间盘退变的超微结构变化。方法:24只新西兰大白兔,随机分组骨性手术组(16只)和软组织手术组(8只)。骨性手术组完整切除L4、L5双侧下关节突,L4-5、L5-6为实验组椎间盘,L3-4、L6-7为自身对照组椎间盘。软组织手术组仅剥离L3至L7的椎旁肌肉,L4-5、L5-6为实验对照组椎间盘。术后1、2、4及8月行电子显微镜检查。结果:术后4月,各组椎间盘开始出现退变细胞,表现为外形不规则,细胞膜破裂,线粒体肿胀,核浓缩,核膜皱缩,异染色质较正常增加,核仁消失。退变细胞数量以实验组椎间盘最多。术后8月,实验组椎间盘开始出现大量死亡细胞,表现为溶酶体明显增多,细胞核扭曲,内质网扩张,线粒体空泡化。结论:关节突关节破坏能够诱发出椎间盘退变的超微结构改变。
Objective： To observe ultrastructure change of degenerated intervertebral disk caused by rabbit bilateral zygapophysial joints destruction. Mothods： 24 male New-Zealand rabbits were randomly divided into operation group on the bone and operation group on the soft tissue. For operation group on the bone, L4 and L5 inferior articular processes were en bloc excised, L5 and L6 superior articular processes were retained. L4-5 and intervertebral disks acted as experimental group; L3-4 and L6-7 acted as self-control group. For operation group on the soft-tissue, Only L3 to L7 paravertebral muscles was stripped. L4-5 and L5-6 acted as experimental control group. The 1st, 2nd, 4th and 8th months after operation, ultrastructure observation of intervertebral disks were performed. Results： At the end of the 4th month post-operation, many degenerative cells were found in the study group, with the features of irregular cell contours, swelling chondrosome, rough endoplasmic reticulum, and condense nucleus located in the cellular nucleus. Eight months after operation, many dead cells were found. Cytolysosomes increased, cellular nucleus became twisted, rough endoplasmic reticulums swelled, and chondrosome became vacuolization. Conclusions： Ultrastructure changes of degenerated intervertebral disk can be induced by destroying bilateral zygapophysial joints of New-Zealand rabbit.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2008年第2期185-188,共4页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金资助项目(30471746)
广东省科委攻关项目(C30702)
关键词
椎间盘
退变
电子显微镜
intervertebral disk
degeneration
electronmicroscope
