摘要
目的探讨用高效液相色谱(HPLC)法测定参仙乙肝灵片的芍药苷含量。方法色谱柱为HypersilODSC18柱(100mm×4.6mm,5μm),流动相为甲醇-水(30∶70),检测波长为230nm,柱温为25℃,流速为1.0mL/min。结果芍药苷进样量在0.068～0.680μg范围内与峰面积线性关系良好(r=0.9999),平均回收率98.61%,RSD为1.02%。结论HPLC法简便、快速,测定结果准确,重现性好,可作为参仙乙肝灵片的质量控制方法。
Objective To establish a HPLC method for determining the contents of paeoniflorin in Shenxianylganling Tablets. Methods Stationary phase was Hypersil ODS C18 （100 mm×4.6 mm,5 μm）,the mobile phase was methanol-water （30：70）.The detection wavelength was 230 nm. The flow rate was 1.0 mL/min and the column temperature was 25℃. Results There was good linearity in the range of 0. 068 - 0.68 μg, r = 0. 999 9（ n = 6） for paeoniflorin. The average recovery rate of paeoniflorin was 98.61% and RSD was 1.02%. Conclusion This method is simple,fast and accurate for the quality control of the preparation.
出处
《中国药业》
CAS
2008年第7期24-25,共2页
China Pharmaceuticals
