摘要
目的:探讨survivin在鼻型NK/T细胞淋巴瘤中的表达,并进一步分析其表达与caspase-3基因表达和细胞增殖的关系。方法:应用免疫组化EnVision System检测survivin、caspase-3和ki-67在50例鼻型NK/T细胞淋巴瘤和12例正常淋巴结组织石蜡标本中的表达,并分析肿瘤组织中survivin与caspase-3基因表达、ki-67增殖指数的关系。结果:肿瘤组织中survivin阳性表达率为60%(30/50),正常淋巴结组织中survivin阳性表达率为16.7%(2/12),且主要局限于少数生发中心细胞,两者差异有显著性(P<0.05);caspase-3在肿瘤组织和正常淋巴结中的阳性表达率分别为84%(42/50)和100%(12/12),但两者差异无显著性统计学意义(P>0.05)。survivin高表达与caspase-3基因表达下调密切相关(P<0.05)。survivin阳性组ki-67增殖指数(38.23±16.54)%明显高于survivin阴性组(23.15±21.53)%(P<0.05)。结论:在鼻型NK/T细胞淋巴瘤组织中,survivin可能通过抑制caspase-3的激活而发挥其抑制细胞凋亡的作用,survivin与细胞增殖指数呈正相关,可能促进肿瘤细胞增殖。survivin异常表达在鼻型NK/T细胞淋巴瘤的发生发展中有一定的作用。
Objective： To investigate the expression of survivin in nasal-NK/T cell lymphoma, and to analyze the relationship of the expression with caspase-3 and cell proliferation. Methods： We detected the expressions of survivin, caspase-3 and ki-67 by immunohistochemical EnVision System in 50 cases of nasal-NK/T cell lymphoma and 12 normal lymph nodes, and analyzed the correlation of the survivin gene in the tumor tissues with the caspase-3 gene and ki-67 proliferation. Results： The positive expression rate of survivin was 60% （30/50） in the tumor tissues and 16.7% （2/12） in the normal lymph nodes, with significant differences between the 2 groups （ P 〈 0.05 ）, while that of caspase-3 was 84% （42/50） and 100% （12/12）, respectively, with no statistic difference in between （P 〉0.05 ）. The ki-67 proliferation index was significantly higher in the survivin-positive lymphomas （38.23 ＋ 16.54） % than in the survivin-negative ones. The high expression of the survivin gene was closely correlated with the down-regu- lation of the caspase-3 gene. Conclusion ： survivin may prevent cell apoptosis by inhibiting the activity of caspase-3 play an important role in the carcinogenesis of NK/T cell lymphomas by promoting cell proliferating.
出处
《医学研究生学报》
CAS
2008年第3期242-244,248,I0002,共5页
Journal of Medical Postgraduates
基金
江苏省六大人才高峰重点课题基金资助项目(批准号:2005A3)
