摘要
目的比较瑞氏-姬姆萨染色法和巴氏染色法对精子形态染色的效果,寻找可替代巴氏染色法的简单、有效的精子形态染色方法。方法随机选取21份精液标本,每份标本同时采用瑞氏-姬姆萨染色法和巴氏染色法对精液涂片进行染色,分析两种染色方法的结果。结果两种染色方法对精子形态的评估结果无显著性差异(P>0.05);且对正常形态精子和异常形态精子的评估分别具有正相关性,r值为0.984、0.986(P均<0.01)。结论瑞氏-姬姆萨染色法是一种简单有效的精子形态染色方法,可替代巴氏染色法。
Objective To Compare Wright-Giemsa stain with Papanicolaou stain on sperm morphology evaluation, and to find a relatively simple and effective sperm morphology stain method which can replace Papanicolaou stain. Methods 21 sperm samples were stained synchronously by Wright-Giemsa and Papanicolaou staining and the results of the two methods were analysed. Results These two stain methods had no difference in evaluating the sperm morphologyand the normal morphology and teratosperrnia（P〉0. 05）. They had a positive correlation, and the correlation coefficient were 0. 984,0. 986 respectively（all P〈 0. 01）. Conclusion Wright-Giemsa stain was convenient, economic, and effective method which in evaluating the sperm morphology. It could replace Papanicolaou stain to assess the sperm morphology.
出处
《华中医学杂志》
CAS
2007年第6期436-438,共3页
Central China Medical Journal
基金
汕头市重点科学技术项目(No200525)
关键词
精子
瑞氏-姬姆萨染色
巴氏染色
Spermatozoa Wright-Giemsa stain Papanicolaou stain
