摘要
目的:探讨p14ARF表达与不同HPV感染状态的外阴鳞癌发生和发展的关系。方法:采用RT-PCR和蛋白印迹技术检测42例外阴鳞癌组织、10例正常外阴组织中p14ARF基因mRNA及蛋白表达水平,PCR技术检测其HPV-DNA,分析HPV阳性组和阴性组外阴鳞癌组织中,p14ARF基因mRNA及蛋白表达与其临床病理特性的关系。结果:外阴鳞癌组p14ARF基因mRNA及蛋白表达强度为0.92±0.24和1.09±0.11;正常外阴组分别为0.93±0.06和1.02±0.38,两组比较差异无统计学意义,P>0.05;低、中和高分化鳞癌组p14ARF基因mRNA表达分别为0.98±0.11、0.94±0.15和0.91±0.07;p14ARF基因蛋白表达低、中和高分化分别为1.14±0.24、1.11±0.27和1.07±0.29,3组间比较差异均无统计学意义,P>0.05。p14ARF基因mRNA及蛋白在HPV阳性组表达强度分别为0.99±0.04和1.11±0.02,HPV阴性组表达强度分别为0.61±0.07和0.76±0.06,两组比较差异有统计学意义,P<0.05。结论:p14ARF可能参与了外阴鳞癌的发生发展过程,并可能成为HPV感染的外阴鳞癌发生进展相关的基因。
OBJECTIVE： To investigate the relationship between the expression of p14^ARF and the development and progression of vulvar squamous cell carcinoma （VSCC） with different HPV infection status. METHODS： Forty-two cases of VSCC and 10 cases of normal vulvar tissues were surveyed for p14^ARF mRNA and protein by RT-PCR and Western-blot, respectively. HPV-DNA was tested by PCR technology, and then the relationship between the expression of mRNA and protein of p14^ARF and its clinical pathological characters in the HPV positive and negative groups of VSCC was investigated. RESULTS： The relative expression content of p14^ARF mRNA and protein were 0. 92±0. 24 and 1.09 ± 0. 11 respectively in VSCC, and 0.93 ± 0.06 and 1.02±0.38 in normal vulvar tissues. There was no significant difference between VSCC and normal vulvar tissue groups （P〉0.05）. There was no significant difference among the poorly differentiated （0.98 ± 0.11, 1.14 ± 0.24）, moderately differentiated （0.94 ± 0. 15, 1.11 ± 0.27） and well differentiated （0.91 ± 0.07, 1.07±0.29） groups （P〉0.05）. The expression of mRNA and protein of p14^ARF in the HPV positive group and HPV negative group were 0. 99±0. 04, 1.11±0. 02 and 0. 61±0.07, 0.76±0. 06 respectively, and there was a significant difference between the two groups （P〈0. 05）. CONCLUSION： p14^ARF may take part in the development and progression of VSCC, and it is also a gene involved in the development and progression of HPV-positive VSCC probably.
出处
《中华肿瘤防治杂志》
CAS
2008年第5期325-328,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
辽宁省自然科学基金资助项目(20042067)
辽宁省教育厅攻关计划项目(2004D178)
