摘要
[目的]建立测定舒必利血药浓度的HPLC方法,并研究Beagle犬单次口服舒必利的药动学过程。[方法]采用岛津LC-10AVP系列HPLC仪,InertsilCN-3(250mm×4.6mm,5μm)色谱柱,以CH3OH-0.025Mol/LKH2PO4=45︰65为流动相,盐酸普奈诺尔作内标。样品在弱碱性条件下用二氯甲烷漩涡混合萃取后挥干进样,荧光检测器检测(Ex=298nmEm=345nm)。[结果]血浆标准曲线在20～3200ng·ml-1范围内有良好线性,且精密度、方法回收率、萃取回收率、稳定性均符合要求。经时血药浓度数据用DAS程序进行药动学拟合,表明舒必利的体内过程符合一室开放模型。[结论]本测定方法灵敏、简便、特异性好,可用于舒必利血药浓度测定和药动学研究。同时对Beagle犬单次口服舒必利的药动学过程作了描述性分析,求出了主要药动学参数。
[ Objective] To develop a RP-HPLC method for the determination of sulpiride in beagle dog＇s plasma and to study the pharmacokinetics. [ Methods] The plasma sample was extracted with 3ml CH2Cl2, then separation was achieved on the Inertsil CN-3 （250mm×4.6mm, 5μm） column. The mobile phase consisted of CH3OH-0.025mol/L KH2PO4 （45 ： 65） and the flow rate was 1ml·min^-1 The samples were measured at fluorescence of Ex=295nm, Ex=302nm. Propranolol Was used as internal standard. [ Results] The calibration curves were linear in the range of 20-3 200 ng· ml^-1. The methodology recovery rate, extraction recovery rate, degree of precision and stability were all qualified. The concentration-time data of sulpiride were treated with DAS pharalacokinetic software and were fitted as a single-compartment open model. [Conclusion] The assay is simple, rapid and sensitive. It is satisfactory, to be used on study of sulpiride.
出处
《现代预防医学》
CAS
北大核心
2008年第7期1322-1323,1327,共3页
Modern Preventive Medicine
关键词
舒必利
血药浓度测定
药动学
BEAGLE犬
Sulpiride
Plasma concentration determination
Pharmacokinetics
Beagle dog
