摘要
目的:探讨磷脂酰肌醇3激酶/蛋白激酶B信号通路是否介导结缔组织生长因子促人增生性瘢痕成纤维细胞转分化。方法:体外培养人增生性瘢痕成纤维细胞,实验分3组:①空白对照组;②结缔组织生长因子(10ng/ml)刺激组;③磷脂酰肌醇3激酶抑制剂LY294002(10μmol/L)预处理后结缔组织生长因子刺激组。应用免疫印迹技术检测48h后a-平滑肌肌动蛋白的表达,应用流式细胞术检测a-平滑肌肌动蛋白阳性细胞百分率。结果:和空白对照组相比,结缔组织生长因子刺激组细胞a-平滑肌肌动蛋白表达和阳性细胞百分率升高;经磷脂酰肌醇3激酶抑制剂LY294002预处理后,再以结缔组织生长因子刺激,细胞a-平滑肌肌动蛋白表达和阳性细胞百分率升高水平下降,经统计学分析,差别具有显著性(P<0.05)。结论:结缔组织生长因子能够促进人增生性瘢痕成纤维细胞转分化,磷脂酰肌醇3激酶/蛋白激酶B信号通路介导该效应,磷脂酰肌醇3激酶抑制剂LY294002可抑制此效应。
Objective To explore if the effect of connective tissue growth factor on the transdifferentation of human hypertrophic scar fibroblasts is mediated by phosphatidylinositol 3-kinase/protein kinase B (PI3K/PKB) signal pathway. Methods Human hypertrophic scar fibroblasts were cultured in vitro. The cells were divided into three groups: ①control group, ②connective tissue growth factor (10 ng/ml)stimulated group, ③PI3K inhibitor LY294002 (10μmol/L)pretreated and connective tissue growth factorstimulated group. The expression of a-smooth muscle actin was examined by western blot in human hypertrophic scar fibroblasts after 48 hours. The positive ratio of a-smooth muscle actin was examined by flow cytometry. Results Compared with the control group, the expression and the positive ratio of a- smooth muscle actin in the connective tissue growth factor stimulated group increased. The raise level of a-smooth muscle decreased after the pretreatment of PI3K inhibitor LY294002. The results were markedly different by statistic analysis (P 〈0.05). Conclusion Connective tissue growth factor can promote the transdifferentation of human hypertrophic scar fibroblasts mediated by PI3K/PKB signaling pathway. This effect can be inhibited by LY294002.
出处
《中国美容医学》
CAS
2008年第3期395-397,共3页
Chinese Journal of Aesthetic Medicine
基金
国家自然科学基金资助项目(编号:30400472)