摘要
为分析离子束介导大豆DNA获得的小麦高蛋白、低蛋白和雄性不育变异株系中性蛋白水解酶和过氧化物酶的变化,采用复性电泳技术对变异株系幼苗的根系、叶鞘和叶片进行了分析。结果显示:(1)在根中检测到的蛋白水解酶带型差异较大、酶活性强;高蛋白变异株系中检测到28、45、55和100 kD四条新带;在雄性不育变异株系中检测到113 kD一条新带;在叶鞘中,所有的变异株系都没有检测到100 kD的酶带。(2)过氧化物酶在根中检测的酶带数较多、酶活性强;在低蛋白变异株系的叶片中检到31 kD一条新带;在叶鞘中,对照和变异株系间酶活性也有一定的差异。(3)小麦幼苗期蛋白水解酶和过氧化物酶从叶片到根系酶带增多、活性增强。由此推测,外源大豆DNA导入受体后某些片段可能插入受体基因组,导致基因突变或受体基因表达发生改变。
High-protein, low-protein and male sterile mutant wheat lines were obtained by transferring the total DNA of bean into wheat Xinmai 9 by ion beam. Organs of wheat seedling of the mutation lines and Xinmai 9 were analyzed by comparing the patterns of renaturation electrophoresis of neutral proteinases and POD isoenzymes. Results indicated： （1）Types and activity of proteinases in roots were stronger and had more difference than in leaves and vaginas. Some new bands, such as 28,45,55 and 100 kD in high-protein mutant, 113 kD in male sterility mutant,were detected. 100 kD wasn＇t detected in the all vaginas of mutant. （2） As to POD, few differences were detected in the leaves and vaginas of those mutants. A new band of 31 kD was detected in high-protein variant line. Some differences on the POD activities between wild type and mutants were also observed. （3） Activities of proteinases and prioxidases showed more and more stronger in leaves and roots in wheat seedling. Based on above results, a conclusion may be inferred that some bean DNA fragments were inserted into the genome of wheat and lead to the mutation of some genes.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2008年第2期325-328,共4页
Journal of Triticeae Crops
基金
河南省自然科学基金资助项目(0511020600)
