摘要
目的:研究补肾壮骨胶囊对去卵巢大鼠骨质疏松症的骨密度和细胞因子等的影响,探索该药对绝经后骨质疏松症的防治功效及作用机理。方法:将大鼠随机分为6组:假手术组、卵巢切除组、切除卵巢+牡蛎碳酸钙组、切除卵巢+补肾壮骨胶囊低剂量组、切除卵巢+补肾壮骨胶囊中剂量组、切除卵巢+补肾壮骨胶囊高剂量组,观察骨密度、血清E2和IL-6、子宫指数和股骨指数等指标,了解其对去卵巢大鼠骨骼的影响。结果:1)骨密度的变化:去卵巢16周后,卵巢切除组大鼠右股骨骨密度和骨矿含量均明显低于假手术对照组,差异有显著性(P<0.01),表明造模成功。与卵巢切除组比较,补肾壮骨胶囊中剂量组和高剂量组可明显提高模型大鼠股骨BMC含量(P<0.05),高剂量组可明显提高模型大鼠股骨BMD的含量(P<0.05)。2)血清E2和IL-6的变化:去卵巢16周后,卵巢切除组大鼠血清E2含量显著低于假手术对照组,差异有显著性(P<0.05)。中药中剂量组及高剂量组E2含量明显高于模型组,差异有显著性(P<0.05)。3)子宫指数和股骨指数:与假手术组比较,卵巢切除组子宫指数和股骨指数明显降低,具有统计学意义(P<0.05),提示造模成功。与卵巢切除组比较,高剂量组子宫指数明显增加(P<0.05);高、中、低三个剂量组股骨指数均明显增加(P<0.05)。结论:补肾壮骨胶囊能增加去卵巢大鼠子宫湿重,但对干重无明显影响,可以显著提高去卵巢大鼠离体骨骨密度及改善其生物力学性能,提高去卵巢大鼠血清E2和IL-6含量,具有类性激素样作用。
Objective：To study the effect of Kidney-Tonifying Bone-Strengthening Capsule on bone mineral density and cytokine in osteoporotic ovariectomized （OVX） rat models, confirming this medicine has preventive and therapeutic effect on postmenopausal osteoporosis （PMOP）. Methods：Sprague Dawley rats, female, were randomly divided into six groups： sham operation group, ovariectomized group, ovariectomized ＋ oyster calcium carbonate group, ovariectomized ＋ low dose capsule group, ovariectomized ＋ middle dose capsule group, ovafiectomized ＋ high dose capsule group, to observe bone mineral density and serum biochemical markers, and study the effect of ovariectomy on bone. Results：l. Change in bone density： 16 weeks after ovariectomy, the bone density and bone mineral content of the rat＇ s fight hind leg thighbones in ovarieetomized group were obviously lower than those of the sham operation group （P 〈 0.01 ）, indicating the model was successfully established. Compared with ovariectomized group, the middle- and the high-dose groups can significantly increase the level of BMC （P 〈 0.05） of the said organ, meanwhile the latter can obviously increase the BMD level （P 〈 0.05）. 2. Change in serum E2 and IL-6 ： 16 weeks after ovariectomy, the serum E2 level in the ovariectomized group was significantly lower than that of the sham operation group （P 〈 0.05 ）, indicating the model was successfully established. The levels in the middle- and high-dose groups were higher than that of the sham operation group （P 〈0.05）. 3. Change in uterus index and thighbones index： Compared with the sham operation group, the indices in ovariectomized group were obviously lowered （P 〈 0.05）, indicating the model was successfully established. Compared with ovariectomized group, the uterus indexes in the high dose group was increased, the thigh- bones index in high-, middle-and low-dose groups were all significantly increased. Conclusion：the Capsule can increase the wet weight of ovarie
出处
《世界中医药》
CAS
2008年第2期110-112,共3页
World Chinese Medicine
基金
澳门特别行政区科学技术发展基金项目
编号:089/2005/A
