摘要
目的通过运用模具加水浴方法制备肝气郁结证候动物模型。方法将大鼠分为空白对照组、模型组、自复组、反证组4组。运用模具加水浴方法造成大鼠肝郁证胃肠运动功能障碍动物模型。测量各组动物的体重、腹围、自发活动、智力、胃和小肠运动功能变化,观察胃肠组织形态学变化,测定血浆胃动素(MTL)、胃泌素(Gas)、生长抑素(SS)、血管活性肠肽(VIP)指标研究肝郁证大鼠胃肠激素的变化。结果模型组大鼠体重增加减慢,腹围指数变大,自发活动减少,智力下降;胃残留率增加、小肠推进率减慢;Gas、MTL和VIP的含量降低,SS的含量升高。用四逆散治疗后上述指标恢复正常。结论用模具加水浴方法制备肝郁证动物模型接近中医肝气郁结证的发病学因素,其模型的情志改变呈现肝郁证典型的临床特点。
Objective To establish the rats model of Liver-Qi depression syndrome with the gastroenteric motional function imbalance. Method The rats were divided into four groups： control, model, resume and disproof. This animal model was made by the method of confining the rat movement, intelligence and the change of the gastroenteric motional function, the gastroentefic histomorphological were observed and analysed, Hormone change of the rats model of Liver-Qi depressionwas studied by measuring the gastroenteric hormone. Results The symptoms of the rats model were as followings ： the falling body weight and intelligence, the reducing movement; the residue solid left in the stomach increases unusually, the drive rate of small intestine became slower; the plasma level of motilin, gastrin and vasoactive intestinal polypeptide decreases, the somatastin improved. Conclusion The stable rat model of Liver-Qi depression syndrome with the gastroenteric function imbalance is estanblished. The rat model presents typical clinic characteristic of the liver-Qi depression syndrome.
出处
《四川动物》
CSCD
北大核心
2008年第2期287-291,共5页
Sichuan Journal of Zoology
基金
国家自然科学基金项目
批准号:39970928
关键词
肝郁证
胃肠运动功能障碍
动物模型
liver-qi depression syndrome
gastroenteric motional function imbalance
rats model
