摘要
转Chi基因小麦的T_1、T_2及T_3代植株的遗传分析、抗白粉病鉴定和考察外源基因影响植株农艺性状的结果表明,T_1和T_2代中外源Chi基因的分离比均接近3:1,符合孟德尔遗传规律,Southern blot检测外源基因均为单拷贝整合。导入Chi基因的小麦对白粉病田间混合菌种的抵抗能力增强。除了株高和生育期以外,转基因植株的其他性状与未转基因的植株基本上相同。
In the text, genetic analysis and powdery mildew resistant identification had been done among T1, T2 and T3 of transferred wheat （Triticum aestivum） with Chi gene. The results indicated that segregation ratio of exogenous Chi gene between T1 and T2 was approximately 3：1, which was consistent with Mendelism. Exogenous genes were found that they all single-copy integration by Southern blot. The Chi gene transferred wheat had a better counteraction against powdery mildew which caused by mixed bacteria in field. Other characteristics of transgenic plant, excluding plant height and period of duration, were all the same as common one＇s.
出处
《植物生理学通讯》
CSCD
北大核心
2008年第1期65-68,共4页
Plant Physiology Communications
基金
哈尔滨师范大学科研基金(KM2006-07)
黑龙江省重大攻关课题(GA068103-10)。
