摘要
目的:探讨羟甲基戊二酰辅酶A还原酶抑制剂(HCRI)氟伐他汀对糖尿病小鼠肾脏白介素-6(IL-6)表达的影响。方法:以链脲佐菌素(STZ)诱导的CD-1小鼠糖尿病动物模型为研究对象。STZ腹腔注射60mg/(kg·d),6～7天。6天后测血糖,血糖≥11.2mmol/L为模型。造模成功后糖尿病小鼠随机分为氟伐他汀干预组5组(每组n=10),连续干预1～3个月,其中干预1个月组分为低剂量[5mg/(kg·d)]、中剂量[25mg/(kg·d)]和高剂量[125mg/(kg·d)]组,干预2个月和3个月组均为中剂量,同时设糖尿病非干预组3组(每组n=10)和正常对照组3组(每组n=8)。分别于干预1个月、2个月、3个月末处死相应各组小鼠,处死前收集尿标本,测血糖,记录小鼠体重,一侧肾重,留取血标本、肾组织。血清检测胆固醇、甘油三酯、尿素氮,尿液检测尿白蛋白/肌酐比值。ELISA法检测肾皮质中IL-6水平。结果:中剂量氟伐他汀连续干预2个月和3个月时,尿白蛋白/肌酐、血尿素氮、肾重/体重比值及IL-6水平均明显低于非干预组(P均﹤0.05),而在整个干预期间,血胆固醇及血甘油三脂水平各组间无显著差异(P>0.05)。结论:氟伐他汀可抑制糖尿病小鼠肾脏IL-6水平的表达,降低蛋白尿,其肾保护作用独立于降脂以外且在一定剂量范围内呈时间依赖性。
Objective：To prohe effects of Fluvastatin （a kind of HCRI） on the expression of IL-6 hy kidney of mice suffering from diabetes mellitus. Methods：Mice induced diahetes mellitus using Streptozotocin （STZ） were used in this trial. The mice were abdominally injected STZ 60 mg/（kg·d） for 6-7 days. The mice that had a blood sugar ≥ 11.2 mmol/L six days later were included in ensuing procedures. The included mice were randomized into 5 Fluvastatin intervention groups （n = 10 for each group） ,continuously undergoing the trial for one,two and three months. The mice that underwent one month trial were subdivided into three groups receiving Fluvastatin with low dosage[5 mg/（kg·d）] ,medium dosage[25 mg/（kg·d）] and high dosage[125 mg/（kg·d）] ,and mice that underwent two and three months trial merely received medium dosage. Three non-intervention groups （n = 10 for each group） and 3 normal con trol groups（n = 8 for each group） were also installed. The mice were sacrificed at the end of first, second and third month after having been collected for urine samples and tested for blood sugar. Mouse body weight, weight of one kidney, blood sample and renal tissue were recorded or stored. The mouse sera were tested for cholesterol,triglyceride,urea nitrogen,and mouse urines were tested for ratio of urinary albumin versus creatinine. ELISA was used to detect level of IL-6 in renal cortex. Results：Compared with mice in non-intervention groups, mice that received medium-dosage of Fluvastatin for two or three months significantly lowered their ratio of urinary albumin versus creatinine,blood urea nitrogen, ratio of kidney weight versus body weight （P 〈 0.05） and level of IL-6 （P〈 0.05）, while cholesterol and triglyceride in blood of the mice remained unchanged （P 〉 0.05）. Conclusion：Fluvastatin was able to inhibit expression of IL-6 in kidney of mice suffering from diabetes mellitus and reduce urinary protein. Meanwhile,Fluvastatin could protect kidney with a time-depend
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期282-285,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅社会发展基金(H200317)
江苏省卫生厅“135”项目基金(苏卫科教[2001]31号文)
