摘要
目的观察三七对酒精性肝病的防治作用。方法70只SD雄性大鼠随机分为正常组10只,模型组、三七高剂量组、三七低剂量组和硫普罗宁组各15只;以56度红星二锅头5g/(kg.d)、玉米油2ml/(kg.d)和吡唑27.2mg/(kg.d)每天1次灌胃,连续14周建立酒精性肝病模型,同时分别以1.2g/(kg.d)和0.6g/(kg.d)的三七粉和100mg/(kg.d)的硫普罗宁灌胃进行干预,均连续14周。全自动生化仪测定各组大鼠血脂、血清肝功能水平,ELISA法检测透明质酸(HA)、层黏蛋白(LN)含量,常规HE及Masson染色光镜观察肝组织的脂肪变、炎症及纤维化程度。结果模型组大鼠肝组织脂肪变及炎症程度、血清CHOL、HDL-C、LDL-C、AST、ALT、HA、LN水平均较正常组明显增高(P<0.01,P<0.05),但血清TG水平则无明显升高(P>0.05)。三七高低剂量组、硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清AST、ALT、血清HA、LN水平较模型组明显减轻(P<0.01,P<0.05),但血清TG、CHOL、HDL-C、LDL-C水平与模型组比差异无显著性差(P>0.05)。结论(1)采用白酒-玉米油-吡唑混合液灌服大鼠14周能成功复制ALD模型,表现肝组织脂肪变和炎细胞浸润、血脂紊乱、血清肝功能及肝纤维化指标升高。(2)三七可明显减轻酒精性肝病大鼠肝组织脂肪变和炎症程度,改善血清肝功能和纤维化指标。
Objective To characterize the effects of Sanchi on the alcoholic hepatopathy rats. Methods 70 SD male rats were randomly divided into five groups： normal group （ n = 10） , control group （n = 15 ） , high - dose Sanchi group （ n = 15 ） , low - dose Sanchi group （n = 15） , and Tiopronin group （n = 15） . In order to induce alcoholic hepatopathy, animals in the control, high -dose and low dose Sanchi, and Tiopronin groups were orally dosed with HongXing ErGuoTou （56% ethanol;5g/kg） , corn oil （2ml/kg） , and pyrazole （27.2 mg/kg） every morning for 14 weeks. Rats in high -dose Sanchi, low dose Sanchi, and Tiopronin groups, were treated every afternoon during the 14 - week induction of hepatopathy, with 1.2g/kg Sanchi, 0.6g/kg, and 100mg/kg Tiopronin, respectively. All rats were sacrificed 12 hours following the last injection. Blood samples were obtained prior to euthanization. Blood - fat and serum lipoidase activity were measured using an automatic blood biochemical analyzer. Blood haluronic acid （ HA ） , laminin （ LN ） were determined by ELISA. Histopathological changes of hepatic tissues （i. e. steatosiss, inflammation, and fibrosis） were assessed by microscopic examination of HE and Masson staining of the right lobe of livers. Results Compared with rats from the normal group （ treated with ddH2O） , rats in the control group have significantly higher steatosis, inflammation, liver fibrosis, and increased level of serum cholesterol, HDL -C, LDL- C, haluronic acid, laminin and AST/ALT activity, （P 〈0.01, P 〈0.05） , while serum TG levels were comparable between the normal and control group（ P 〉 0.05 ）. More importantly, treatment with high - dose, low - dose Sanchi, or Tiopronin resulted in less severe steatosis, inflammation, fibrosis in the liver, and reduced the levels of serum haluronic acid, laminin （P 〈0.01 , P 〈0.05） , while serum TG, cholesterol, HDL - C, LDL - C levels remained largely unchanged relative to untreated rats （P 〉 0.05 �
出处
《医学研究杂志》
2008年第3期35-38,F0003,共5页
Journal of Medical Research
基金
浙江省中医药科技计划项目(2006Y018)
杭州市医药卫生科技计划项目(2005B071)
关键词
酒精性肝疾病
三七
动物模型
Alcoholic liver diseases
Sanchi
Animal model
