摘要
目的:探讨天龙咳喘灵胶囊(TLC)对慢性常压低氧性肺动脉高压大鼠的防治作用。方法:24只SD大鼠随机分为:正常对照组、单纯低氧组和低氧+TLC组,观察肺组织形态学变化,测定各组大鼠平均肺动脉压(mPAP)、体循环平均压(mSAP)和右室肥厚指数(RVH I);计算动脉中膜厚度占外径的百分比(MT%)、管壁面积占血管总面积的百分比(WA%)和管腔面积占血管总面积的百分比(LA%);检测动脉血中是一氧化氮NO含量和一氧化氮合酶NOS活性。结果:(1)与正常对照组相比,低氧组大鼠肺组织切片HE和弹力纤维染色可见肺小动脉壁增厚、管腔狭窄和肺泡隔增厚等改变,TLC干预后可显著减轻低氧所致的肺组织病理改变;低氧组mPAP、RVH I、MT%和WA%显著高于正常对照组(P<0.01),而低氧+TLC组与正常对照组相比差异无统计学意义(P>0.05);低氧组LA%与正常对照组和低氧+TLC组相比显著减小(P<0.01)。3组mSAP差异无统计学意义(P>0.05)。(2)慢性低氧使大鼠动脉血中NO含量和NOS活性显著降低(P<0.01),而低氧+TLC组与正常对照组相比差异无统计学意义(P>0.05)。结论:TLC可抑制大鼠慢性低氧性肺动脉高压的形成,其机制可能与其增强NOS活性、增加NO含量有关。
Objective： To investigate the preventive effect of Tianlongkechuan capsules （TLC） on chronic hypoxic-induced pulmonary hypertension in rats. Methods： Twenty-four Sprague-Dawley rats were randomly divided into Control （ C group）, Hypoxia （ H group）, and Hypoxia ＋ TLC groups （ HT group）. Morphology of pulmonary tissue and blood vessels, mean pulmonary arterial pressure （mPAP）, mean systemic artery pressure （mSAP） and right ventricle hypertrophy index （RVHI） were evaluated. Percentages of intimea-media thickness to the external diameter （MT%） ,wall area to total transectional area （WA%） and luminal area to total transectional area （LA%） were calculated. NO concentration and NOS activity in blood serum were measured. Results： （1） Microstructure of pulmonary tissue and pulmonary arteries of H group changed obviously with the development of hypoxia-induced pulmonary vascular structural remodeling, and those changes of HT group were ameliorated. （2） mPAP and RVHI of H group [ （ 19.41 ± 3.68 ） mmHg and （ 31.05 ± 5.42 ） ] were significantly higher than those of C group [ （ 14.28 ± 2.09 ） mmHg and （20.78 ± 1.90） ] and HT group [ （ 15.30 ± 2.64） mmHg and （ 22.92 ± 4.25 ） ] （ P 〈 0.01 ）. There was no significant difference in mSAP among the three groups （ P 〉 0.05 ）. （3） Plasma NO concentration and NOS activity of H group [ （84.25 ± 13.31 ） μmol/L and （ 16.51 ± 3.89 ） U/L ] were markedly decreased compared with those of C group [ （ 105.59 ± 6.95 ） μmol/L and （28.35 ± 1.80 ） U/L ] and HT group [ （ 116.53 ± 15.19 ） μmol/L and （ 24.03 ± 3.54 ） U/L] （P 〈 0. 01 ）. Conclusion： These results indicate that TLC may have a preventive effect on chronic hypoxiainduced pulmonary hypertension, which may be related to enhanced NOS activity and alteration of NO level.
出处
《广州医学院学报》
2007年第4期9-12,共4页
Academic Journal of Guangzhou Medical College
基金
广州市卫生局科研项目(编号2004A058)
关键词
天龙咳喘灵胶囊
高血压
肺性
低氧
一氧化氮
一氧化氮合酶
Tianlongkechuan capsule （TLC）
hypertension, pulmonary
hypoxia
nitric oxide
nitric-oxide synthase
