摘要
目的研究32080株链球菌蛋白体外对5-氟尿嘧啶（5-FU）引起的小鼠脾细胞增殖抑制及凋亡的影响并探索其相关机制。方法NIT法检测链球菌蛋白和5-FU对小鼠脾细胞增殖活性的影响；流式细胞仪分析链球菌蛋白和5-FU对脾细胞周期分布、凋亡率及线粒体膜电位等的影响。结果不同浓度的链球菌蛋白（10～100μg/mL）能显著促进脾细胞增殖，并可拮抗5-FU所致的脾细胞增殖抑制作用；链球菌蛋白（50μg/mL）能明显促进5-FU阻滞的脾细胞通过S期，进入G2／N期，恢复细胞的有丝分裂进程；链球菌蛋白（50μg/mL）明显拮抗5-FU引起的脾细胞线粒体膜电位下降（P〈0．01），降低5-FU引起的脾细胞凋亡率（P〈0．01）。结论链球菌蛋白对小鼠脾细胞具有明显的增殖活化作用，拮抗5-FU对细胞的增殖抑制作用，促进5-FU抑制的脾细胞进入细胞分裂周期，稳定其线粒体膜电位，抑制细胞线粒体途径凋亡。
Objective To study the effects of streptococcal protein of 32080 strain on the growth inhibition and apoptosis of murine splenocytes induced by 5-fluorouracil （5-FU） in vitro, and probe its possible mechanisms. Methods The effects of streptococcal protein and 5-FU on the proliferation activity of murine splenocytes were determined by MTr method. The distribution of the cell cycle, the apoptotic rate, and mitochondrial membrane potential of murine splenocytes caused by streptococcal protein and 5-FU were analyzed by flow cytometry analysis. Results Streptococcal protein （ 10 - 100 μg/mL） could increase the proliferation of splenocytes and antagonize the growth inhibition of splenocytes induced by 5-FU significantly. Streptococcal protein （50μg/mL） could promote splenocytes from S phase to G2/M phase in cell cycle obviously and recover the progression of cell mitosis. Streptococcal protein （50 μg/mL） could antagonize the decrease of mitochondrial membrane potential（ P 〈 0. 01 ） and diminish the cell apoptotic rate induced by 5-FU （ P 〈 0. 01 ）. Conclusion Streptococcal protein can activate murine splenocytes, antagonize the cell growth inhibition caused by 5-FU, promote the progression of cell cycle, stabilize the mitochondrial membrane potential, and inhibit apoptosis by the mitochondrial-dependent pathway.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2008年第2期130-134,共5页
Immunological Journal
基金
北京市教委科技发展计划资助项目(KM200410025008)
关键词
链球菌
5-FU
脾细胞
细胞周期
细胞凋亡
Streptococcus
5-Fluorouracil
Murine splenocyte
Cell cyde
Apoptosis
