摘要
目的研究慢性萎缩性胃炎(CAC)胃黏膜表皮生长因子(EGF)、血管内皮生长因子(VEGF)及增殖细胞核抗原(PCNA)与中医证型的关系,探讨中医证型的实质,为临床治疗提供理论依据。方法经胃镜及病理组织学检查确诊的200例CAG患者,进行中医辨证分型,采用免疫组化法检测不同证型CAG患者EGF、VEGF及PCNA的表达水平。结果脾胃虚弱证72例,肝胃不和证43例,脾虚气滞证32例,胃阴不足证24例,脾胃湿热证14例,胃络瘀阻证5例。其中,PCNA表达水平肝胃不和证与脾胃虚弱证、脾虚气滞证、胃阴不足证之间差异有统计学意义(P<0.05);而EGF、VEGF的表达水平在各型之间差异无统计学意义(P>0.05)。结论CAG以脾胃虚弱证和肝胃不和证居多,PCNA的高表达可能是肝胃不和证的诊断依据。
Objective To study the relationship of TCM syndrome type of gastric mucosal epithelial growth factor （EGF）, vascular endothelial growth factor （VEGF） and proliferative cell nuclear antigen （PCNA） in patients with chronic atrophic gastritis （CAG） for exploring the essence of TCM type and providing a theoretical basis of clinical treatment. Methods TCM syndrome type of 200 patients with diagnosis of CAG confirmed by fibro-gastroscope and pathological examination were differentially classified, and the expressions of EGF, VEGF and PCNA in different types were determined using immunohistochemistry. Results Patients were differentiated as Pi-Wei deficiency type （Type Ⅰ ） in 72; Gan-Wei disharmony type （Type Ⅱ ） in 43; Pi-deficiency with qi stagnation type （ Type Ⅲ ） in 32 ; Wei-yin deficiency type （ Type Ⅳ ） in 24 ; Pi-Wei damp-heat type （ TypeⅤ ） in 14 ; and Weicollateral stasis obstruction type （Type Ⅵ） in 5. The difference of PCNA expression level between Type Ⅱ with Type Ⅰ , Ⅲ and IV was significant （ P 〈 0. 05）. No significant difference in expression levels of EGF and VEGF was found among the 6 types （ P 〉 0.05）. Conclusion Type Ⅰ and Ⅱ were the dominant TCM syndrome types in CAG patients; the high expression of PCNA might be a diagnostic evidence for Gan-Wei disharmony syndrome.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第3期225-228,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
山东省中医管理局科研基金项目(No.2005-237)
关键词
慢性萎缩性胃炎
中医证型
免疫组织化学
表皮生长因子
血管内皮生长因子
增殖细胞核抗原
chronic atrophic gastritis
TCM syndrome type
immunohistochemistry
epithelial growth factor
vascular endothelial growth factor
proliferative cell nuclear antigen
