摘要
报道了在ＹＷＧＣ１８柱上，以甲醇＋水（７．５＋９２．５）、含５ｍｍｏｌ／ＬｐＨ５．０磷酸盐缓冲溶液、１０ｍｍｏｌ／ＬＴＢＡ·Ｂｒ作流动相，同时分离尿嘧啶、９Ｎ二羟丙基腺嘌呤、腺嘌呤、可可碱、尿嘧啶丙酸、９Ｎ二羟丙基茶碱和咖啡因，并用于茶叶中咖啡因的测定，检出限为μｇ／Ｌ级，标准加入回收率为９５．３％～１０５．０％。
Uracil,9 N dihydroxypropyladenine,adenine,theobromine,1 propionyloxyuracil,9 N dihydroxypropyltheophylline,and caffeine were separated on a column of YWG C 18 by use of methanol+water(7.5+92.5) containing 5 mmol/L of pH 5.0 phosphate buffer solution and 10 mmol/L of TBA·Br as mobile phase.Caffeine in tea has been determined by this method with a detection limit of μg/L order of magnitude and a recovery rate of 95.3%～105%.
出处
《分析测试学报》
CAS
CSCD
1997年第3期17-20,共4页
Journal of Instrumental Analysis
基金
湖北省自然科学基金
关键词
反相离子对
嘌呤
嘧啶
茶叶
咖啡因
HPLC
碱基
Reversed phase ion pair HPLC, Purine compound, Pyrimidine compound, Tea, Caffeine.
