摘要
目的研究肝细胞癌(HCC)中5-脂氧合酶(5-LOX)的表达与bcl-2和bax表达的关系及其可能机制。方法检测48例HCC癌组织及其癌旁组织,应用RT-PCR和免疫组织化学S-P法检测5-LOX、bcl-2和bax的表达情况。结果在癌、癌旁组织中5-LOX、bcl-2和bax基因mRNA表达分别是30/48、33/48、24/48和12/48、9/48、36/48,与癌旁组织比较,差异有显著性(P<0.05)。HCC中5-LOX蛋白阳性率为56.3%,与bcl-2蛋白的表达呈显著正相关(r=0.447,P<0.01),但与bax蛋白表达呈显著负相关(r=-0.567,P<0.01)。结论5-LOX与肝细胞肝癌的发生、发展关系密切;其机制可能是通过上调bcl-2及同时下调bax等凋亡相关调节因子表达水平有关。
Objective To study the relationship between the expression of 5-1ipoxygease（5-LOX）, bcl-2 and bax in hepatocelluar carcinoma（ HCC）, and to investigate its possible mechanisms. Methods The expression of 5-LOX, bcl-2 and bax was detected by RT-PCR and immunohistochemical method with 48 cases of HCC . Results 5-LOX, bcl-2 and bax were observed in 30/48, 33/48, 24/48 and 12/48, 9/48 and 36/48 in hepatocellular carcinomas and adjacent non-cancerous tissues, respectively, mRNA of 5-LOX, bcl-2 and bax genes in hepatocellular carcinoma increased significantly compared with adjacent non-cancerous tissues （P 〈 0. 05）. 5-LOX expressed in 56. 3% of 48 HCC cases, which was positively correlated with bcl-2 expression （r =0. 447, P 〈0. 01-）, but negatively correlated with bax （ r = - 0. 567 P 〈 0. 01 ）. Conclusion 5-LOX might play a significant role in the happen and the development of HCC cells via up-regulating bcl-2 expression and down-regulating bax expression.
出处
《肝胆外科杂志》
2008年第1期24-27,共4页
Journal of Hepatobiliary Surgery
