摘要
应用合酶链反应（ＰＣＲ），核酸分子杂交和病毒分离技术检测了７例临床诊断为病毒性脑炎患者及１０例其他神经疾病对照组患者的脑脊液（ＣＳＦ）中单纯疱疹病毒（ＨＳＶ）ＤＮＡ。结果ＰＣＲ检出５例脑炎患者ＣＳＦ中ＨＳＶＤＮＡ阳性，其中ＨＳＶ１型（ＨＳＶ－１）２例，ＨＳＶ２型（ＨＳＶ－２）３例，而核酸杂交方法仅检出２例，其中ＨＳＶ－１和ＨＳＶ－２各１例，与病毒分离法结果一致。ＰＣＲ技术在无菌性脑炎的诊断上具有更高的敏感性和特异性，因而较为实用。实验还比较了２种ＤＮＡ模板制备方法，煮沸法简便快速，更宜推广。
7 CSF specimens from. clinical definate viral encephalitis patients and 10 from patients with other neurological diseases were examined for herpes simplex virus infection by means of polymerase chain reaction ( PCR) , DNA hybridization and virus isolatioh.Among the viral encephalitis secimens, 5 were shown HSV DHA positive by PCR, 2 of themwere firmed to be HSV-linfection and 3 were HSV-2.Only 2 were shown by DNA hybridization and virus isolation, Result suggested PCR may be more reliable for the diagnosis of viral encephlitis be- cause of its relative higher sensitivity and specificity Two DNA Extraction methods for prepa- ration of PCR DNA template. from CSF had been compared, CSF Boiling method showed more advantages than. Phenol-Chloroform Extraction.
出处
《赣南医学院学报》
1997年第2期102-105,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
关键词
聚合酶链反应
病毒性脑炎
单纯疱疹病毒
CSF
Polymerase chain reaction Viral encephalitis Herpes simplex virus DNA hybridization Virus isolation .
