摘要
【目的】探讨慢性乙型肝炎和慢性重型乙型肝炎患者外周血单核细胞Toll样受体2(TLR2)的变化情况及其意义。【方法】随机选取慢性乙型肝炎患者31例,设定为慢性乙型肝炎组,慢性重型乙型肝炎患者30例设定为慢性乙型肝炎组,健康志愿者30例为正常对照组,用流式细胞仪检测各组外周血单核细胞表面Toll样受体2的表达,ELISA法检测血清肿瘤坏死因子α(TNF-α)的水平,荧光定量PCR法检测血清HBV DNA并取对数作为复制水平的指标,总胆红素(TB)和凝血酶原时间(PT)的检测按照本院常规检测。三组均数间的比较采用单因素方差分析和Scheffe检验进行两两间的比较;两组计量资料间的比较采用Mann-WhitneyU秩和检验;两组数据间的相关性分析采用线性相关分析;P<0.05为有统计学意义。【结果】正常对照组、慢性乙型肝炎组及慢性重型乙型肝炎组外周血单核细胞TLR2表达强度和外周血清TNF-α水平均分别为(21.5±2.7)MFI、(39.0±4.1)MFI、(47.7±21.4)MFI和(54±38)ng/L、(164±90)ng/L、(360±140)ng/L,依次逐渐升高,且各组间比较均具有显著性差异;外周血单核细胞TLR2表达水平与血清TNF-α表达水平呈现显著的正相关。慢性重型乙型肝炎组与慢性乙型肝炎组TB,PT和血清HBV DNA对数值分别为(470±225)μmol/L与(101±131)μmol/L,(35.1±21.1)s与(15.4±2.2)s和5.9±2.0与5.4±1.6;慢性重型乙型肝炎组与慢性乙型肝炎组比较,TB、PT显著升高而血清HBV DNA对数值无显著性差异;外周血单核细胞TLR2表达水平与TB及PT呈现显著的正相关,但与血清HBV DNA对数值间无显著的相关性。【结论】TLR2可能与慢性乙型肝炎及慢性重型乙型肝炎的发病有关。
[Objective] To study the change of TLR2 on peripheral blood monocytes （PBMCs） and its role in the pathogenesis of chronic and chronic severe hepatitis B. [Methods] Randomly selected 31 patients with chronic hepatitis B, 30 patients with chronic severe hepatitis B and 30 healthy controls entered into this study. The expression of TLR2 on PBMCs was determined by flow cytometry. The level of serum tumor necrosis factor α （TNF-α） was determined by ELISA. The serum HBV DNA level was determined by fluorescence PCR and presented as Lg （HBV DNA）. Total bilirubin （TB） and prothrombin time （PT） were routine tests. The differences between three means were determined by one-way ANOVA and Scheffe test. The difference between two means was determined by Mann-Whitney U test. The correlations between TLR2 and TNF-α, Lg （HBV DNA）, TB, PT were determined by linear correlation test. [Results] The values of TLR2 on PBMCs and serum TNF-α in the group of healthy controls, the group of patients with chronic hepatitis B and the group of patients with chronic severe hepatitis B were （21.5 ± 2.7）MFI, （39.0 ± 4.1）MFI, （47.7 ± 21.4）MFI, and （54 ± 38）ng/L, （164 ± 90） ng/L, （360 ± 140）ng/L. Both of them increased gradually and significantly. There was a significant positive correlation between the value of TLR2 and the value of serum TNF-α. The values of TB, PT and Lg（HBV DNA） in the group of patients with chronic severe hepatitis B and the group of patients with chronic hepatitis B were （470 ± 225） μmol/L and （101 ± 131） μmol/L, （35.1 ± 21.1）s and （15.4 ± 2.2）s, 5.9 ± 2.0 and 5.4 ± 1.6. The values of TB and PT in the group of patients with chronic severe hepatitis B were significantly higher than those in the group of patients with chronic hepatitis B. But there was no significant difference for the values of Lg（HBV DNA） between the above two groups. There were significant positive correlations between the values of TLR2 and TB, PT, but not Lg（HBV DN
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2008年第1期91-94,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金(05001710)
