摘要
在云南富民、砚山、邱北和南涧等云南主要辣椒产区采集了28个病毒样品，选择应用RT-PCR，MspⅠ以及EcoRⅠ酶切和克隆测序等分子生物学手段对其中的7种进行检测。经过RT-PCR实验确定其病原为黄瓜花叶病毒（CMV），同时分离物的MspⅠ酶切图谱与CMV亚组Ⅰ的酶切图谱很相似，经EcoRⅠ酶切后也没有出现异常差异。进一步对所检测的黄瓜花叶病毒分离物的外壳蛋白基因进行克隆测序，结果表明，分离物的核苷酸序列与黄瓜花叶病毒亚组Ⅰ的分离物的核苷酸序列有极高的同源性，达93％-98％；而与亚组Ⅱ株系的核苷酪南列同源性仅为77％。因此将所分离到的黄瓜花叶病毒分离物归属于黄瓜花叶病毒亚组Ⅰ。
Twenty-eight capsicum disease samples were collected from the main producing areas of Yunnan, including Fumin, Yanshan, Qiubei and Najian. Seven of them were detected by RT-PCR, the PCR products were digested by Msp Ⅰ and EcoR Ⅰ to get 7 isolates and the coat protein genes of these isolates were cloned and sequenced. The results were as follows ： （ 1 ） the result of RT-PCR showed that the disease was caused by cucumber mosaic virus （CMV） ; （2） the electrophoresis bands of Msp Ⅰ digestion showed that the isolates was very similar to the isolates from subgroupⅠ ; （3） the electrophoresis bands of EcoR Ⅰ digestion had no distinct difference. （4） seven coat protein genes shared 93% - 98% homology with those of CMV subgroup Ⅰ strains from Genbank and shared only 77% homology with those of CMV subgroup Ⅱ strains. It was determined that all of seven CMV isolates belonged to the subgroup Ⅰ of CMV.
出处
《云南农业大学学报》
CAS
CSCD
2008年第2期167-172,共6页
Journal of Yunnan Agricultural University
基金
云南省科技攻关项目(2006NG02)
关键词
黄瓜花叶病毒
辣椒
亚组
cucumber mosaic virus （CMV）
capsicum
subgroup
