摘要
目的:通过测定哮喘患者外周血及外周血单个核细胞(PBMC)受特异性过敏原刺激后细胞因子IFN-γ/IL-4、转录因子T-bet/GATA-3的表达变化,探讨Th1、Th2与过敏性哮喘之间的关系。方法:①采用ELISA法检测发作期过敏性哮喘患者血浆IFN-γ/IL-4及PBMC在粉尘螨(Df)刺激下产生IFN-γ/IL-4的水平,并以正常人为对照。②采用半定量RT-PCR反应检测过敏性哮喘患者PBMC受过敏原刺激前后T-betmRNA、GATA-3mRNA的表达水平,并以正常人为对照。结果:①哮喘患者血浆IL-4水平较正常人升高(P=0.0017),IFN-γ与正常人无明显差异(P=0.7600),IFN-γ/IL-4的比值显著低于正常人(P=0.0001)。②经过敏原刺激后,哮喘患者PBMC产生的IL-4、IFN-γ水平均较正常人显著升高(t=3.5644,P=0.0010;t=3.3500,P=0.0020),但哮喘患者IFN-γ/IL-4的比值仍显著低于正常组(t=6.3640,P=0.0001)。③哮喘患者PBMC中GATA-3的表达量较正常人显著增高(t=2.2743,P=0.0280),而T-bet的表达量与正常人比较无显著变化(t=0.3823,P=0.7044),哮喘患者T-bet/GATA-3比值与正常人也未见显著差异(t=1.1152,P=0.2834)。④经过敏原刺激后,哮喘患者PBMC中T-bet、GATA-3表达水平较正常人显著增高(t=2.2982,P=0.0276;t=3.7887,P=0.0006),但T-bet/GATA-3的比值与正常人比较仍无显著差异(t=1.1959,P=0.2491)。结论:在细胞因子及转录因子水平上,过敏性哮喘患者不仅存在着Th2反应增强,也存在着Th1反应的增强,因此用Th1/Th2平衡失调学说尚不能满意解释过敏性哮喘的发病机制。
Objective：To identify the contributions of allergen to Th1 and Th2 by determined the levels of IFN-γ,IL-4 and the transcription factors T-bet and GATA-3 in peripheral blood mononuclear cells（PBMC）of allergic asthmatics.Methods：①Plasma IFN-γ and IL-4 were measured in 35 asthmatics and 32 healthy by ELISA,as well as the production of IFN-γ and IL-4 by Dermatophagoides farinae（Df）-activated PBMC in asthmatics and 15 healthy controls.②Twenty-five asthmatics and 15 healthy persons were enrolled.The T-bet mRNA and GATA-3 mRNA expressing levels in PBMC were assayed by one step reverse transcription polymerase chain reaction（RT-PCR）.Results：①The plasma IL-4 in the patients was significantly higher than that in healthy（t=3.265 9,P=0.001 7）.The plasma IFN-γ levels in asthmatics were no significantly different from that of the healthy（t=0.306 8,P=0.760 0）.② In the condition of PBMCs activated by Df,the IL-4 and IFN-γ levels in asthmatics were increased compared with healthy（t=3.564 4,P=0.001 0;t=3.350 0,P=0.002 0）.③ There were some expression of the transcription factors T-bet and GATA-3 of PBMC in asthmatics and healthy.The expression of GATA-3 in the patients was significantly higher than that in healthy（t=2.274 3,P=0.028 0）.The expression of T-bet and the ratio of T-bet/GATA-3 in the patients was no significant difference with the healthy（t=0.222 0,P=0.825 8;t=1.115 2,P=0.283 4）.④ In the Df-activated PBMC,the expression of T-bet and GATA-3 in asthmatics were significantly higher than that of healthy（t=2.298 2,P=0.027 6;t=3.788 7,P=0.000 6）,but the ratio of T-bet/GATA-3 was no significant difference with healthy（t=1.195 9,P=0.249 1）.Conclusion：The production of Th2,Th1 cytokines and the expression of GATA-3 and T-bet in allergic asthmatics are both increased.It is suggested that the imbanlance of Th1/Th2 unsatisfactorily explain the pathogenesis of allergic asthma.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第2期179-183,187,共6页
Chinese Journal of Immunology
