摘要
目的了解血管内皮生长因子(VEGF)、胰岛素样生长因子Ⅱ(IGF-Ⅱ)在肝细胞癌(HCC)中的诊断意义。方法分别用酶联免疫吸附测定(ELISA)法及电化学发光免疫测定(ECLIA)法测定40例HCC、50例肝硬化(LC)及50例正常对照者(NC)血清VEGF、IGF-Ⅱ、甲胎蛋白(AFP)水平。结果HCC患者血清VEGF(432.40±296.70)ng/L、IGF-Ⅱ(0.80±0.29)μg/L的水平明显高于正常对照组(129.40±58.70)ng/L(P<0.01)、(0.38±0.08)μg/L(P<0.01)及LC组(149.50±89.10)ng/L(P<0.05)、(0.61±0.12)μg/L(P<0.01),二者与AFP联合检测可提高HCC诊断阳性率达94.3%。结论AFP与VEGF和IGF-Ⅱ联合检测敏感度高,三者联合检测更具有优越性,可以提高肝癌的检出率,避免漏诊,具有重要的临床意义。
Objective To study the clinical significance of serum vascular endothelial growth factor（VEGF） and insulin-like growth factor-Ⅱ （IGF-Ⅱ） assay in the diagnosis of hepatocellular carcinoma （HCC）. Methods Serum VEGF,IGF-Ⅱ and alpha-fetoprotein（AFP） concentrations were determined in 40 patients with HCC, 50 patients with liver cirrhosis （LC） and 50 normal controls by means of enzyme linked immunosorbent assay and electrochemiluminescence immunoassay respectively. Results The levels of serum VEGF and IGF-Ⅱ of HCC patients （432.40±296.70） ng/L, （0.80±0.29）μg/L were significiantly higher than those in normal controls （129.40±58.70） ng/L（P〈0.01）,（0.38±0.08）μg/L（P〈0.01）,LC patients （149.50±89.10） ng/L（P〈0.05）,（0.61±0.12） μg/L（P〈0.01）. The total positive detection rate of HCC with combined VEGF, IGF-Ⅱ and AFP reached to 94.3%. Conclusion The total positive rate HCC with combined VEGF,IGF-Ⅱ and AFP is high. The sensitivity rate of AFP combined detection with VEGF and IGF-Ⅱ is superior,it can improve the diagnosis of HCC and avoid missing diagnosis with important clinical significance.
出处
《临床荟萃》
CAS
北大核心
2008年第5期325-327,共3页
Clinical Focus
关键词
肝肿瘤
血管内皮生长因子A
甲胎蛋白类
生长调节素类
liver neoplasms
vascular endothelial growth factor A
alpha-fetoproteins
somatomedins
