摘要
目的研究多药耐药相关蛋白2(MRP2)基因及MRP3、MRP5基因在正常肝脏细胞系L-02、肝癌细胞系BEL及肝癌耐药细胞系BEL/ADM的表达差异,并探讨其在肝癌耐药中的意义。方法分别提取L-02、BEL及BEL/ADM细胞的总RNA,利用实时荧光定量PCR法检测MRP2、MRP3、MRP5基因的表达水平。结果MRP2基因在BEL/ADM细胞中的表达量明显高于在L-02、BEL细胞中的表达(P=0.000),而其在L-02、BEL两种细胞中的表达没有显著性差异(P=0.468);MRP3、MRP5基因表达量在L-02、BEL、BEL/ADM三种细胞中均有显著性差异(P=0.000)。结论MRP2可能参与了肝癌的内在性耐药,而MRP3、MRP5可能与肝癌的获得性耐药有关。
Objective To investigate the differential expression of genes encoding multidrug resistance-associated protein 2 （MRP2）, MRP3 and MRP5 in human normal liver cell line L-02, hepatocarcinoma cell line BEL and its adriamycin-resistant counterpart BEL/ADM cell line. Methods The total RNA was extracted from L-02, BEL and BEL/ADM cells, respectively, and the mRNAs were reversely transcribed into cDNA. The expression levels ofMRP2, MRP3, MRP5 mRNAs were detected according to the standard curve using real-time fluorescence quantitative PCR. Results The expression of MRP2 mRNA in BEL/ADM cell line was significantly higher than that in L-02 and BEL cells （P=0.000）, and the latter two cells showed similar expression levels ofMRP2 mRNA （P=0.468）. The expressions of MRP3 and MRP5 mRNA showed statistically significant difference between the 3 cell lines （P=-0.000）. Conclusion MRP2 might play a role in the intrinsic drug resistance, and MRP3 and MRP5 are related to the acquired drug resistance ofhepatocellular carcinoma.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第2期219-221,224,共4页
Journal of Southern Medical University
基金
广东省自然科学基金(020097)~~
关键词
肝肿瘤
多药耐药相关蛋白
实时荧光定量PCR
liver neoplasms
multidrug resistance-associated protein
real-time fluorescence quantitative polymerase chain reaction
