摘要
本文用膜片籍技术（细胞贴附式和内面向外式）研究了孕烯酮硫酸酯（PS）对大脑皮层锥体神经元膜48pS延迟整流型钾通道（Ik）开放概率和平均开放时间的影响。在加入PS后40s，即可观察到Ik通道的开放概率增大，平均开放时间延长，表明PS加强Ik通道的活动。在浓度为30～100μmol／L的范围内，其增大程度与PS的浓度呈正相关，但PS不影响通道的电流幅度。本文为阐明孕激素快速膜效应的机制提供了新的资料。
Recent studies have shown that endogenous brain metabolites of steroids may exertimportant nongenomic modulatory effects on neuronal mechanisms. In this work the effect of pregnenolone sulfate (PS) on delayed rectifier K+ channel (Ik) was studied inacutely dissociated rat cerebral cortical pyramidial neurons by means of eell-attachedand inside-out patch clamp techniques. The open probability and mean open time of theIK channels were increased by PS in a concentration-dependent manner over the rangeof 30~100μmol/L. but without changing the IK amplitude.
出处
《生理学报》
CAS
CSCD
北大核心
1997年第4期407-413,共7页
Acta Physiologica Sinica
基金
国家自然科学基金!39370281
关键词
孕烯酮硫酸酯
锥体神经元
钾通道
大脑皮层
pregnenolone sulfate
pyramidial neurons
potassium channel
patch clamp
