摘要
目的观察骨保护素(OPG)、骨保护素配体(OPGL)在燃煤型氟中毒大鼠骨组织中的表达水平,探讨燃煤型氟中毒对大鼠骨保护素、骨保护素配体表达的影响。方法以SD大鼠为研究对象,按体重均衡随机分为5组(组内雌雄各半):高氟组、高氟偏食组、低氟组、低氟偏食组、对照组。以氟病区煤烘玉米为主要饲料,复制氟中毒动物模型。用免疫组织化学方法检测OPG、OPGL在大鼠胫骨干骺端的表达。结果(1)各染氟组大鼠干骺端OPG表达增高,与对照组比较差异有统计学意义(P<0.05)。高氟组与低氟组比较,OPG表达增高,差异均有统计学意义(P<0.05)。(2)各染氟组大鼠干骺端OPGL表达增高,与对照组比较差异有统计学意义(P<0.05)。高氟偏食组与高氟组、低氟偏食组与低氟组比较,OPGL增高,差异有统计学意义(P<0.05)。结论过量氟造成骨与破骨活动均增强的骨转换增高状态。氟可通过改变OPG和OPGL相对表达的比例影响骨吸收的程度。低钙偏食可促进氟骨症破骨吸收增强,与过量氟对骨骼引起骨转换增强的作用具有同向性。
Objective To observe the expression of OPG,OPGL in bone of fluorosis rats caused by coal burning and to explore the effects of skeletal fluorosis and osteoprotegerin and steoproteger in ligand.Methods SD rats taken as the experlmental objects were randomly assorted into 5 groups（the number either female or male was the same in each group）：control group,high-dose fluoride group,high-dose fluoride group-emented with low calcium diet group,low-dose fluoride group,low-dose fluoride supplemented with low calcium diet group.All rats were fed with corn dried by burning coal,which came from the borough with high prevalence of fluoride,to establish the animal model of fluorosis.The tibia slices of chronic fluorosis rots were stained vith HE;OPG、OPGL in tibia metaphysis were observed by immunohistochemistry.Results Compared with control group,the number of OPG increased in every test group and the difference was statistically significant（P〈0.05）.Compared with high-dose fluoride group and low-dose fluoride group the increase of OPCL expression in high-dose fluoride supplemented with low calcium diet group and low-dose fluoride supplemented with low calcium diet group was statistically significant（P〈0.05）.The compact bone of the cortex of high-dose fluoride supplemented with low calcium diet group become porosity.Conclusion Excessive fluoride can induce accelerative bone turnover and enhance the activity of osteoclastic absorption by increasing the OPCL.Low calcium diet can promote bone absorption of skeletal fluorosis.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2008年第3期327-329,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(00260099)
关键词
煤
氟中毒
骨保护素
骨保护素配体
coal
fluorosis
steoprtegerin
osteprotegrin ligand
bone
