摘要
用荧光方法观察了松材线虫(Bursaphelenchus xylophilus)携带的细菌对悬浮培养的黑松(Pinus thunber-gii)细胞的毒性,表明其中的假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性,这与松树发病早期出现的细菌种类一致;这些细菌在添加松树提取液的细菌培养基上不能生长或基本不能生长,表明松树体内可能存在某些不利于细菌生长的物质;同时,单独接种这些细菌不能感染健康的黑松,因此,在线虫最初进入树体中,细菌的生长与线虫密切相关.
By fluorescent observation of pine wood nematode-carrying bacteria toxicity to black pine suspending culture cell, culture filtrates of Pseudomonas sp. , P. fluorescence and Sphingomonas sp. were observed strong toxic effects to black pine cell. These bacteria species are coincide with those in the early stage of pine wilt disease. All these bacteria can totally not grow or grows badly on the media incorporating the extracts of pine trees, indicating some substances existing in the trees will harmful to the bacterial strains, which is further convinced by inoculation showing that single bacterial species will not infect Pinus thrgbergii. Hence, the growth and reproduction of the nematode-carrying bacteria is closely related to the nematodes,when they first enter to the trees.
出处
《苏州大学学报（自然科学版）》
CAS
2008年第1期80-84,共5页
Journal of Soochow University(Natural Science Edition)
基金
国家自然基金重点项目(30430580)
关键词
松材线虫
黑松
细菌
致病菌
bursaphelenchus xylophilus
pinus thunbergii
bacteria
pathogenicity
