摘要
为了解单采浆供血员庚型肝炎病毒（ＨＧＶ）感染及其转归，对１０２名抗－ＨＣＶ阳性单采浆供血员冻存血清进行抗－ＨＧＶ和ＨＧＶＲＮＡ检测，对抗－ＨＧＶ和（或）ＨＧＶＲＮＡ阳性者作３年随访研究。采用ＥＩＡ法检测抗－ＨＧＶ，包被抗原来自ＨＧＶ不同功能区的合成肽。应用ＲＴ－ＰＣＲ法检测ＨＧＶＲＮＡ，引物选自ＨＧＶＮＳ３区。结果表明，抗－ＨＣＶ阳性单采浆供血员ＨＧＶＲＮＡ阳性率为１９．６１％（２０／１０２），抗－ＨＧＶ阳性率为１７．６５％（１８／１０２），ＨＧＶ感染率（抗ＨＧＶ和／或ＨＧＶＲＮＡ阳性）为２４．５１％（２５／１０２），而对照组仅为０．９４％（１／１０６）。提示单采血浆是ＨＧＶ感染的重要危险因素。ＨＧＶＲＮＡ和抗－ＨＧＶ的３年阴转率分别为３５．００％（７／２０）和１１．１１％（２／１８）。
Antibodies against hepatitis G (anti HGV) and HGV RNA were detected for frozen stored sera collected from 102 plasma donors and those subjects with positive anti HGV and HGV RNA were followed up for three years to study prevalence of HGV infection in them and their end results. Anti HGV was detected by enzyme immunoassay (EIA) with embedded antigen of synthetic peptide derived from different function regions of HGV. HGV RNA was detected by reverse transcription polymerase chain reaction (RT PCR) with primers derived from NS 3 region of HGV. Results showed that 19.61 percent (20/102) and 17.65 percent (18/102) of the plasma donors were positive for HGV RNA and anti HGV, respectively, and 24.51 percent of them were infected with HGV (either positive for anti HGV or HGV RNA), while only 0.94 percent (1/106) positive in the controls. It suggests that plasma donation is an important risk factor for HGV infection. Negative conversion rates of HGV RNA and anti HGV during three year follow up were only 35.00 and 11.11 percent, respectively (7/20 and 2/18), which indicated a tendency of chronic carriage status in HGV infection.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1997年第5期269-271,共3页
Chinese Journal of Preventive Medicine
