摘要
目的:观察SSB多肽与DNA抗原单独和共同免疫动物时抗体产生的特征。方法:设计合成SSB214￣225aa多肽。用SSB多肽、双链(ds)-DNA、SSB+DNA、血蓝蛋白及PBS分别免疫新西兰白兔,用ELISA法检测SSB多肽抗体,免疫荧光法检测ds-DNA抗体,免疫印迹法检测可提取核抗原(ENA)抗体。结果:ELISA检测显示SSB多肽免疫兔能诱导相应抗体产生,联合免疫组SSB抗体的滴度高于单独SSB免疫组。免疫荧光法检测显示联合免疫组及SSB免疫组各有1只兔产生抗ds-DNA抗体,免疫印迹法检测显示联合免疫组2只兔除产生了抗SSB抗体外,还产生了抗RNP、抗Sm抗体,提示产生表位扩展。结论:合成SSB多肽与DNA共同免疫兔后产生的抗SSB多肽抗体滴度高于单独SSB免疫组,并更易产生表位扩展。
Objective： To observe profile of antibody production in rabbits immunized with synthesized SSB peptides alone or with SSB plus dsDNA. Methods： 214-225 aa peptide of SSB antigen was synthesized. Rabbits were immunized with synthetic SSB peptides linked with keyhole limpet hemocyanin; dsDNA; SSB plus DNA; KLH and phosphate buffered solution （PBS）. SSB peptide antibody was measured by ELISA, ENA antibody and dsDNA antibody were measured by immunoblot and immunofluorescence respectively. Results： Specific anti-SSB peptide antibody was produced after immunization of rabbits with SSB peptide alone or SSB plus dsDNA. The level of SSB peptide antibodies in co-immunization group was higher than that of SSB immunization group. Antibodies against RNP, Sm and/or dsDNA were found in rabbit of co-immunization group. In addition, antibody to dsDNA was also found in rabbits immunized with SSB peptide, suggestive of epitope spreading. Conclusion： Epitope spreading was observed in immunized rabbits especially in those immunized with SSB and dsDNA.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2008年第3期163-165,共3页
Journal of Clinical Dermatology
基金
国家自然科学基金资助项目(30271194)
