摘要
目的:已有研究表明新型生物材料聚磷酸钙掺锶后能明显促进成骨细胞的增殖和分化。实验将掺锶聚磷酸钙与破骨细胞于体外复合培养,进一步从细胞的形态和生理功能两方面考察掺锶聚磷酸钙对破骨细胞的影响。方法:实验于2006-10/2007-01在四川大学组织工程支架材料研究室及四川大学华西医学院生物医学工程重点实验室完成。将机械分离法获得的新生兔长骨破骨细胞分别种植于1cm×1cm×80μm的骨片及由"湿式法"溶液反应法制备的φ2mm×10mm的掺锶聚磷酸钙样品上,以聚磷酸钙为对照,复合培养于24孔培养板中。复合培养7d后,采用特异性抗酒石酸酸性磷酸酶染色方法鉴定实验细胞,通过扫描电镜观察骨片和掺锶聚磷酸钙、聚磷酸钙材料表面破骨细胞的形貌及骨吸收陷窝的形成情况。实验过程中对动物处置符合动物伦理学要求。结果:①抗酒石酸酸性磷酸酶染色显示试验细胞呈阳性。②骨片上形成的骨吸收陷窝多呈不规则形状,陷窝边界清晰,底面粗糙。证实所培养的细胞为破骨细胞。③复合培养7d后,掺锶聚磷酸钙组材料表面陷窝形成的数量明显少于聚磷酸钙组材料。④复合培养7d后,掺锶聚磷酸钙组材料表面破骨细胞的增殖度明显低于聚磷酸钙组材料。结论:掺锶聚磷酸钙由于锶元素的掺入能通过降低破骨细胞的活性,阻碍破骨细胞的增殖分化,从而显著地抑制其骨吸收能力。
AIM： Calcium polyphosphate as a new biomaterial can enhance the replication and differentiation of osteoblasts after being incorporated strontium. In this report, we will conduct a complex culture of strontium-doped calcium polyphosphate （SCPP） with osteoclasts in vitro, and further observe the effect of SCPP on figuration and physiological function of osteoclasts. METHODS： The experiment was performed at the Tissue Engineering Scaffold Materials Laboratory and Huaxi Biomedical Engineering Laboratory of Sichuan University from October 2006 to January 2007. Osteoclasts obtained from new-laid rabbits by mechanical separation were compounded with 1 cm × 1 cm × 80 μm bone slices and φ 2 mm × 10 mm SCPP sample, which was prepared with solution reaction method, in 24-hole culture plate. CPP was used as the control. SCPP and CPP were produced by ＇wet process＇. Seven days after culture, tartrate-resistant acid phosphatase staining was applied to identify the cells. Osteoclast figuration and resorptive pits on bone slice, SCPP and CPP were viewed by scanning electron microscopy. RESULTS： （1）Cells presented masculine after tartrate-resistant acid phosphatase staining.（2）Resorptive pits with clear edge and rough undersurface appeared irregular shape on bone slices. Osteoclasts were identified.（3）Seven days after co-culture, the number of the resorptive pits on surface of SCPP was much smaller than that of CPP. （4）Seven days after co-culture, the proliferation of osteoclasts on surface of SCPP was obviously lower than that of CPP. CONCLUSION： SCPP can remarkably inhibit the osteoclastic bone resorption by reducing its activity, proliferation and differentiation because of the addition of strontium.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第1期19-22,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30370411
50472091)~~
