摘要
对大孔树脂D001分离啤酒废酵母中的还原型谷胱甘肽进行了研究。其最佳试验条件为:pH4.5的0.02mol/L磷酸缓冲液平衡,pH7.5的0.02mol/L磷酸缓冲液洗脱,洗脱流速为2.0mL/min。在最佳分离条件下,大孔树脂D001分离GSH的平均收得率为20.03%,产品中GSH的含量为28.47%,产品颜色为白色。
Macroporous resin D001 was adopted for separation of GSH from waste beer yeast, The result indicated that the optimum concentration of phosphate buffer, pH values for adsorption and elution and the elution rate were 0.02 mol/L, 4.5, 7.5, and 2.9 mL/min, respectively. Under those conditions, the purity of the crude white GSH was 28.47% analyzed by HPLC, and the average yield was 20.03%.
出处
《现代食品科技》
EI
CAS
2008年第2期131-133,共3页
Modern Food Science and Technology
基金
湖北省教育厅重大项目资助(项目编号2003Z001)
关键词
谷胱甘肽
大孔树脂
啤酒废酵母
glutathione
macroporous resin
abandoned beer yeast
