摘要
目的:探讨表皮生长因子(EGF)对激素非依赖性前列腺癌(HRPC)PC-3细胞中内皮素1(ET-1)及其受体mRNA表达的影响。方法:EGF作用不同时间(0、8、16、24、32、48h)后,RT-PCR法测定PC-3细胞中ET-1及其受体ETAR mRNA、ETBR mRNA表达;EGF干预24h后,RT-PCR法测定ET-1及其受体ETAR mRNA、ETBR mRNA表达变化。结果:在PC-3细胞中可检测到ET-1及ETAR mRNA表达,但无ETBR mRNA表达;EGF可上调ET-1及ETAR mRNA表达,与对照组比较,差异具有显著性;ET-1及ETAR mRNA表达随EGF干预时间增加而增加,EGF作用不同时间对PC-3细胞ET-1、ETAR mRNA表达的影响不同,差异具有显著性(P<0.05)。结论:EGF可上调PC-3细胞中ET-1及ETAR mRNA表达,为HRPC的治疗提供了分子生物学基础。
Objective： To investigate the effects of the epidermal growth factor on the mRNA expression of endothelin-1 and its receptors （ETAR, ETBR） in hormone refractory prostate cancer （HRPC） PC-3 cell lines. Methods： PC-3 cells were cultured in vitro. After the treatment with EGF, the mRNA expressions of endothelin-1, ETAR and ETBR were detected by RT-PCR in PC-3 cell lines. The levels of the mRNA expression of endothelin-1 and its receptors were examined at different time points by RT-PCR. Results： The expressions of endothelin-1 and ETA R mRNA but not the mRNA expression of ETB R was observed in PC-3 cell lines. After 24 hours of treatment with EGF, the expressions of endothelin-1 and ETA R in PC-3 cell lines were both up-regulated and there was significant difference （ P 〈 0.05） between the experimental and control groups. Different expression levels of endothelin-1 and ETA R mRNA were noted at different time points of EGF intervention, up-regulated with the increase of treatment time, and with significant difference （ P 〈 0.05）. Conclusion： EGF can up-regulate the mRNA expressions of endothelin-1 and ETA R in PC-3 cell lines and play a great role in prostate cancer progression, which may offer a substructure of molecular biology for the treatment of HRPC.
出处
《中华男科学杂志》
CAS
CSCD
2008年第1期15-19,共5页
National Journal of Andrology
基金
江苏省人类功能基因组学重点实验室开放研究课题基金(HFG007)
