摘要
通过60Co-γ射线处理干稻草秸秆以及提取的稻草粗纤维,研究辐照预处理后的稻草秸秆酶降解效果。采用DNS法和硫酸-苯酚法分别测定处理样品中水溶性还原糖和总糖的含量;通过电子显微镜扫描观测辐照处理对稻草秸秆纤维组织结构的影响。结果表明:采用1 000 kGy^1 500 kGy的辐照剂量处理稻草秸秆,能有效破坏稻草的纤维组织结构,特别是稻草表面硅晶结构和纤维结构,随着辐射剂量的加大破坏程度增强,稻草水溶性还原糖和总糖含量显著增加。辐照与酶复合处理稻草秸秆能极显著地提高稻草秸秆纤维的转化率,稻草秸秆降解的水溶性还原糖含量达到21.44%,总糖含量达75.85%。辐照处理对木质素降解无明显影响。
The water-soluble deoxidize carbohydrate and total carbohydrate of ^60Co-γ, radiated rice straw and raw fibres were measured by DNS method and vitrol-phenol method. The effects of enzyme hydrolyze of the pre-radiated rice straw were studied. The organism structures of radiated rice straw were scanned by electron microscope. It showed that 1 000 kGy - 1 500 kGy ^60Co-γ radiation doses destroy effectively rice straw＇ s organism structures, especially the silicon crystal structures. And along with radiation doses the breakage degree increase prominently. The contents of the water-soluble deoxidize carbohydrate and total carbohydrate of rice straw increase prominently. Treated by both radiation and enzyme, and the cellulose transform rate of rice straw is better than that treated by ^60Co-γ radiation or enzyme. The content of water-solubility deoxidize carbohydrate of the treated rice straw is 21.44 % and the total carbohydrate of straw is 75.85 %. The contents of lignin of the rice straw are not influencing obviously by irradiation pretreatment.
出处
《激光生物学报》
CAS
CSCD
2008年第1期38-42,共5页
Acta Laser Biology Sinica
基金
湖南省科技攻关计划项目(06FJ3188)
关键词
^60CO-Γ辐射
稻草秸秆
纤维
酶水解
还原糖
总糖
^60Co-γ radiation
rice straw
fibre
enzyme-hydrolyze
deoxidize carbohydrate
total carbohydrate
