摘要
目的:考察天然冬虫夏草、蛹虫草及其发酵制品水提物和醇提物减轻自由基引起的人肝癌细胞株SMMC-7721氧化损伤的能力。方法:测定虫草5个抗氧化指标:减轻H2O2对细胞生长的毒害作用,抑制自由基诱导的脂质过氧化,抑制胞内GSH的减少,减轻自由基引起的DNA氧化损伤,降低胞内ROS水平。结果:天然和发酵虫草制品具有不同程度的抗氧化能力,多数情况下其活性与样品加入量呈正比。经综合比较认为天然蛹虫草水提物的抗氧化活性在8个样品中最强,50μg/ml用量时与对照组相比细胞存活率上升90.4%,脂质过氧化值降低51.3%,胞内GSH量升高125%,DNA氧化损伤量降低54.9%,胞内ROS水平减少54.8%。结论:虫草及其发酵制品可作为一种潜在的天然抗氧化剂使用,发酵培养会影响虫草制品抗氧化活性,研究推测虫草抗氧化活性可能来源于它能够降低胞内自由基的量。
Objective： Investigating the protective effects of water and ethanol extracts of natural Cordyceps sinensis and Cordyceps militaris and their fermentation preparations against free radical induced oxidative damage in human hepatoma cell line SMMC-7721. Method： The samples were tested through 5 assays： reducing cell growth suppression caused by I-IzOz; inhibition of free radical induced lipid peroxidation; inhibition of intracellular GSH decrease; inhibition of free radical induced DNA oxidative damage and decreasing intracellular ROS levels. Results： Natural and cultured Cordyceps sp. showed different antioxidant ability, and in most cases, their antioxidant activity was dose dependent. Among all 8 extracts, the aqueous fraction of natural C.militaris indicated the highest activity, the inhibition of cell growth decreased 90.4 %, lipid peroxidation levels decreased 51.3%, intracellular GSH increased 125 %, DNA oxidation damage decreased 54.9%, intracellular ROS levels decreased 54.8% compared with control group at 50 μg/ml. Conclusion： Partial Cordyceps sp. and their fermentation preparations could be regarded as potent natural antioxidants, and the fermented process could affect the antioxidant ability of cultured Cordyceps sp much. The work also presumed that the antioxidant activity of samples might mainly derive from reducing intracellular ROS.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第2期387-390,共4页
Food Science
关键词
抗氧化
冬虫夏草
蛹虫草
发酵
自由基
SMMC-7721
antioxidant
Cordyceps sinensis
Cordyceps militaris
fermentation
free radicah SMMC-7721
