摘要
在GB/T 5009.45-2003国家标准方法的基础上对样品的前处理等过程进行了新的尝试,使样品前处理过程简单、快速,同时保证了方法的准确度和精密度。结果显示,在60℃水浴、30min条件下,三氯乙酸的提取效果最佳;正戊醇提取过程采用旋涡后离心的方式代替了分液漏斗振摇,获得了较佳的提取效果;盐酸提取液改为1.0mL,保证了标准系列与测定系列显色体系的一致性,同时保证了实验结果的准确度。
Exploration of the sample processing was discussed based on GBfF 5009.45-2003, making it simple, and rapid, also assuring methods＇ accuracy and precision. The results showed that the extraction effect of trichloroacetic acid was the optimal at 60℃in 30 min. Using vortex and centrifuge instead of tap funnel in the extraction course of n-pentanol, achieved a good effect on extraction; the extract solution of hydrochloric acid changed to 1.0 mL, guaranteed the colour system＇ consistent between criterion and determined series,and also guaranteed the accuracy of the result.
出处
《食品科技》
CAS
北大核心
2008年第1期223-225,229,共4页
Food Science and Technology
基金
国家科技基础条件平台建设项目(2004DEA70880)
农业部项目委托合同(2005-010)。
关键词
鱼罐头
组胺
测定
分光光度法
canned fish
histamine
determination
Spectrophotometry
