摘要
目的:观察可溶性Tie2融合蛋白(soluble Tie2fusion pro-tein,sTie-Fc)对小鼠脉络膜血管新生(choroidal neovascu-larization,CNV)的抑制作用。方法:采用激光击射的方法诱导成年C57BL/6小鼠CNV模型,术前及术后3,6,9d选取1眼玻璃体腔注入人sTie-Fc0.67μg,对侧眼在相同时点注入人IgG作为对照。激光术后10d处死小鼠,行脉络膜灌流铺片及组织病理学检查观察CNV的发展,定量评价sTie-Fc对于实验性CNV的抑制作用。结果:术后10d所有激光光斑均发展为实验性CNV,脉络膜灌流铺片(16.7±4.0)×10-3mm2vs(30.2±5.1)×10-3mm2,P<0.01)及组织病理学检查(1.70±0.56vs3.07±0.91,P<0.01)结果一致表明sTie-Fc明显抑制了小鼠CNV的发展。结论:sTie-Fc可以抑制实验性CNV的发展。
AIM： To investigate the inhibitive effects of soluble Tie2 fusion protein on choroidal neovascularization （CNV） in mice.
METHODS： CNV was induced in C57BL/6 mouse eyes by krypton laser photocoagulation. Preoperatively and on 3, 6 and 9 days after the photocoagulation, the animals were intravitreally injected 0. 67μg soluble Tie2 fusion protein （sTie-Fc） in one eye, and the fellow eye was injected human IgG as control. The mice were sacrificed at 10 days after laser photocoagulation. Choroidal fluorescein angiography and histopathological examination were used to observe the development of CNV. The inhibition of sTie-Fc on experimental CNV was assessed quantitatively.
RESULTS： Laser-induced CNV developed in all lesions on day 10. The development of CNV was significantly inhibited in sTie-Fc group, indicated by choroidal fluorescein angiography and histopathological examination（ P〈 0.01 ）.
CONCLUSION ： Our findings suggested that Tie2 may be a promising potential target of anti-angiogenesis therapy for CNV.
出处
《国际眼科杂志》
CAS
2008年第2期272-274,共3页
International Eye Science
