摘要
目的:观察Gnb2l1基因及其蛋白在大鼠体内是否存在节律性表达。方法:12D/12L光暗循环下,采用半定量RT-PCR的方法观察大鼠脑组织及肝脏组织的Gnb2l1,Per1 mRNA的变化规律;采用Western Blot方法检测大鼠脑组织及肝脏组织中Gnb2l1编码蛋白、PER1蛋白在不同时间点的变化。结果:12D/12L光暗循环下,大鼠脑组织及肝脏组织中Gnb2l1 mRNA和节律基因Per1mRNA及其蛋白产物均呈近日节律。结论:在大鼠体内,Gnb2l1基因及其编码的蛋白存在着近日振荡,其相位在节律基因Per1之前。由于Gnb2l1基因编码的蛋白RACK1是一种重要的接头分子,参与了PKC信号通路,而Per1基因的启动子又可以被PKC通路激活,加之前期试验证实RACK1能与PER1蛋白相互作用,因此RACK1蛋白很可能在有Per1参与的节律基因分子振荡环路的反馈机制中起到了重要的作用。
Objective： To investigate the circadian rhythm of Gnb2l1 gene and its protein in rat. Method： detecting Gnb2l1 mRNA level with the rat brain and liver samples by RT-PCR under condition of 12D/12L. Observing the expresssion level of RACK1 protein, which was encoded by Gnb2l1 gene, with the rat brain and liver samples by Western Blot under the same condition. Results： Gnb2l1 mRNA levels showed circadian oscillating in rat brain and liver under condition of 12D/12L. the protein, which was encoded by Gnb2l1 gene, also took on a circadian profile. Conclusion： Gnb2l1 gene and its protein take on a circadian profile in rat. so, Gnb2l1 gene involves in the circadian molecular loop, and may play a crucial role in it.
出处
《四川生理科学杂志》
2007年第4期145-147,共3页
Sichuan Journal of Physiological Sciences
基金
国家自然科学基金资助项目(No.30470684
30570902)
