摘要
报道一种分离与定量测定生物合成反应液中谷胱甘肽的方法。该反应以半胱氨酸、甘氨酸和谷氨酸为底物,在葡萄糖、氯化镁和磷酸缓冲液条件下进行,反应液用纸层析分离,实验发现胱氨酸替代半胱氨酸参与反应,并报道了五种成分的R_f值:L-半胱氨酸、甘氨酸、L-谷氨酸、谷胱甘肽和L-胱氨酸。结果表明,在混合溶剂系统中,以酚-水(5:1)分离效果最佳。
A method of separation and quantitative determination of glutathione(GSH) in biosynthesis reaction from its constituent- L-cysteine, glycine and glutamic acid, in a medium containing, glucose, potassium phosphate buffer and MgCl-, by paper chromatography was developed. Cystine was found to play the role of L-cysteine in the biosynthesis of glutathione and Rf values of the 5 components; L-cysteine, glycine, glutamic-acid, GSH and cystine were reported. The results showed that among mixtures, phenol and water mixture in a ratio of (5:1) gave the best separation.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1997年第3期173-179,共7页
Journal of China Pharmaceutical University
关键词
谷胱甘肽
生物合成
纸层析
Glutathione(GSH)
Biosynthesis reaction
Paper chromatography
Analysis determination
