摘要
从河水中驯化、筛选、分离得到一株对染料苯胺蓝有降解能力的菌株WZR-B,该菌能以苯胺蓝为唯一碳源、能源生长.通过对菌株WZR-B的形态特征、生理生化,以及16Sr DNA序列分析,初步鉴定为铜绿假单孢菌(Pseudomonas aeruginosa).研究苯胺蓝的质量浓度、pH值和温度等因素对该菌株生长,以及对降解苯胺蓝的影响.结果表明,菌株生长和脱色的最适pH值为6.5,最适合温度为30℃,苯胺蓝的最适脱色质量浓度为200 mg.L-1.此外,总有机碳(TOC)残留量测定结果表明,该菌株对苯胺蓝的脱色率可达83%,TOC去除率为80%以上,不仅能对苯胺蓝脱色,而且还能进行矿化降解.
A strain WZR-B was domesticated, screened and isolated from a contaminative river, which can utilize aniline blue as the sole carbon and energy source for growth. It was preliminarily identified as Pseudomonas aeruginosa based on morphological, physiological and biochemical features and 16Sr DNA sequence. The effects of dye mass concentration, pH value and temperature on the growth of this strain and its degradation ability was studied. The results suggested that the optimum mass concentration for anline blue decolorization was 200 mg·L^-1 , the optimum pH and temperature for decolorization were 6. 5 and 30℃, respectively. Residual total organic carbon （TOC） analysis suggested that the TOC removal rate was greater than 80% and the aniline blue decolorization rate reached 83%, the strain can not only decolored aniline blue but also mineralized it.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2008年第1期38-41,共4页
Journal of Huaqiao University(Natural Science)
基金
福建省自然科学基金计划资助项目(D0610021)
福建省重大专项前期研究项目(2005YZ1023)
厦门市科技计划项目(3502Z20073009)
关键词
苯胺蓝
生物降解
脱色
假单胞菌
aniline blue
biodegradation
decolorization
Pseudomonas aeruginosa
