摘要
为了探讨济宁青山羊季节性发情的内分泌机理,本试验分别用放射性免疫法和免疫组织化学方法研究了济宁青山羊在春分、夏至、秋分和冬至时血浆内褪黑素的变化规律以及松果体细胞内5-羟色胺(5-HT)的含量变化。结果显示,血浆内褪黑素含量在午夜达到最大值,而在白天日中附近降到最小值,在夜间显著高于白天(P<0.01)。褪黑素含量也存在明显的季节性变化,夏至时夜间的平均含量极显著高于其它3个季节(P<0.01),春分和秋分时差异不明显(P>0.05),但它们显著高于冬至时的夜间平均水平(P<0.05)。白天血浆褪黑素含量在各个季节差异不显著(P>0.05)。夏季5-羟色胺阳性细胞面积和占总面积比值都显著小于其它3个季节,冬季最大,春季和秋季差异不显著(P>0.05)。
For studying the endocrine mechanism about seasonal empathema of Jining Gray goats, the principle of the melatonin concentration in peripheral blood and serotonin contents in pineal body were tested by radioimmunoassay and immunohistochemistry in vernal equinox, summer solstice, autumnal equinox and winter solstice. The results indicated that the concentration of melatonin showed circadian rhythm in peripheral blood, it reached the maximum at midnight and minimum at midday. Melatonin contents associated with seasonal changes. At night, the average concentration of melatonin in the summer solstice was the highest （P〈0.01）, no difference between the vernal equinox and the autumn equinox （P〉0.05）, and the concentration of melatonin in this three seasons were significant higher than that in the winter solstice （P〈0.05）. At daytime, melatonin contents showed no difference in all seasons （P〉0.05）. The area of serotoninpositive cells was the smallest in summer, the largest in winter in pineal body. There were no significant difference between spring and autumn （P〉0.05）.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第2期158-163,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省教育厅资助项目(03H03)
