摘要
目的探讨n-6／n-3多不饱和脂肪酸（polyunsaturated fatty acid，PUFA）不同比例对乳腺癌细胞株MCF-7（ER^＋）及MDA-MB-231（ER^-）胰岛素样生长因子受体一1（insulin—like growth factor receptor-1，IGF-1R）表达的影响。方法用不同比例的n-6／n．3PUFA（1～10）处理细胞，MTF法检测细胞增殖能力变化；RT—PCR检测细胞IGF-1RmRNA表达变化；Westem blot检测IGF-1R蛋白在细胞膜的表达变化。结果单纯n-6PUFA和10：1n-6／n-3 PUFA能够明显促进MCF-7细胞和MDA—MB-231细胞增殖，诱导细胞IGF-1 RmRNA及蛋白表达上调（P〈0．01）；单纯n-3PUFA和1：1n-6／n-3PUFA则显著抑制细胞增殖，并降低细胞IGF-1RmRNA及蛋白表达水平（P〈0．01）；但5：1n-6／n-3PUFA效果不明显（P〉0．05）。结论不同n-6／n-3PUFA构成能差异性调节乳腺癌细胞IGF-1R表达，其中单纯n-3PUFA和1：1n-6／n-3PUFA能明显降低其表达并抑制乳腺癌细胞增殖。
Objective To explore the effects of different n-6/n-3 polyunsaturated fatty acid （PUFA） ratio on the expression of insulin-like growth factor receptor-1 （ IGF-1 R） in breast cancer cells. Methods MCF-7 and MDA-MB-231 cells were treated with n-6 PUFA, or n-3 PUFA, or the mixture of n-6/n-3 PUFA at ratio of 1： 1, 5： 1, 10： 1. The cell proliferation capacity was detected by MTI＇, and the expressions of IGF-1R mRNA and protein were assayed by RT-PCR and Western blot, respectively. Results n-6 PUFA and 10：1 n-6/n-3 PUFA obviously promoted cell proliferation and up-regulated the expression of IGF-1R mRNA and protein, n-3 PUFA and 1：1 n-6/n-3 PUFA greatly repressed cell proliferation and down-regulated IGF-1R mRNA and protein expression. However, 5：1 n-6/n-3 PUFA had no obvious influence both on the cell growth and IGF-1R expression. Conclusion n-6/n-3 PUFA of different ratio has diverse effects on IGF-1R gene expression in breast cancer cells, n-3 PUFA and 1：1 n-6/n-3 PUFA significantly inhibit the proliferation and IGF-1R gene expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第3期195-198,共4页
Journal of Third Military Medical University
基金
国家自然科学基金(30500408)~~
关键词
n-6/n-3
PUFA
乳腺癌细胞
胰岛素生长因子受体-1
n-6/n-3 polyunsaturated fatty acid ratio
breast cancer cell lines
insulin-like growth factor reeeptor-1
