摘要
In order to obtain Pleurotus ferulae with high temperature tolerance, mycelium mono-cells of wild type strain ACK was treated by nitrogen ion (5~30 keV, 1.5×1015~1.5×1016 cm-2) implantation, and mutant CGMCC1762 was selected through auxotrophy screening method, which was Lys, VB6 auxotrophy stress with high temperature. We found that during riper period the surface layer mycelium of the mutant was not aging neither grew tegument even above 30°C. The mycelium endurable temperature of the mutant was increased 7°C compared with that of the wild type strain. The fruiting bodies growth temperature of the mutant was 16~20°C in daytime and was 6~12°C at night. The highest growth temperature of fruiting bodies of the mutant was increased by 5°C than that of original strain. Through three generation investigation, we found that the mutant CGMCC1762 was stable with high temperature tolerance.
In order to obtain Pleurotus ferulae with high temperature tolerance, mycelium mono-cells of wild type strain ACK was treated by nitrogen ion （5-30 keV, 1.5×10^15-1.5×10^16cm^-2） implantation, and mutant CGMCC1762 was selected through auxotrophy screening method, which was Lys, VB6 auxotrophy stress with high temperature. We found that during riper period the surface layer mycelium of the mutant was not aging neither grew tegument even above 30℃. The mycelium endurable temperature of the mutant was increased 7℃ compared with that of the wild type strain. The fruiting bodies growth temperature of the mutant was 16-20℃ in daytime and was 6-12℃ at night. The highest growth temperature of fruiting bodies of the mutant was increased by 5℃ than that of original strain. Through three generation investigation, we found that the mutant CGMCC1762 was stable with high temperature tolerance.
基金
Supported by the National Developing and Reform Committee Project of China [2004]2077
关键词
突变原理
侧耳
高温环境
辐射损伤
Pleurotusferulae, N^＋ beam, High temperature tolerance
