摘要
本文试验了采用原子吸收分光光度计的光路系统测量分子光谱的可行性．在ＰＨ＝３．５的ＨＡＣ－ＮａＡｃ介质中，在避光条件下，钴对无色的Ｆｅ（Ⅲ）－联吡啶配合物还原成红色的Ｆｅ（Ⅱ）－联吡啶配合物有较强的催化作用．在λ＝５２２９ｎｍ处，在原子吸收分光光度计上测定钴的含量，对钴测定的线性范围为３～１５０μｇ／２５ｍｌ，检测限力０．０３８μｇ／２５ｍｌ．本法选择性较好，多数离子允许大量存在，将此法用于维生素Ｂ１２针剂中钴的测定，并与分光光度法及原子吸收分光光度法对照．
This paper descripes a new method for the deiermination of moleculer spectrum by the atomic adsorption spectrophotometer. In the prescence of Co(Ⅱ),color less Fe(Ⅱ)-dipy complex couled be reduced in HAC-NaAC medium(pH3.5) to form a coloured complex in the dark.The coloured complex could be measured by atomic adsorption spectrophotometer.The linear range of Co(Ⅱ) is 3.0～150μg/25ml,and the detection limit is 0.038μg/25ml.The method has been uesd todetermined trace amounts of Co(Ⅱ)in VB 12 sample, and satisfactory results were got complied with atomic adsorption spectrophotometry and UV-Vis spectrophotometry.
关键词
原子吸收
分光光度计
钴
催化光度法
分子光谱
atomic adsorption spectrophotometer cobalt catalytic spectrophotometry
