摘要
目的分析腰椎术后硬膜外前后方外瘢痕组织成熟程度，指导我们预防和治疗腰椎术后硬膜外瘢痕组织的形成。方法选用24只SD雄性大鼠随机按2、4、8、12周分四组，进行后路全椎板切除术。在术后2、4、8、12周时，每组各取6只大鼠进行免疫组化染色，标记α-平滑肌肌动蛋白、纤维连接蛋白、结蛋白。对腰椎术后硬膜外前后方瘢痕组织进行计算机图象分析和统计学处理。结果免疫组化染色定量分析的结果，晚期（8-12周）时硬膜外前方瘢痕组织中α-SMA和FN与后方有显著性差异（P〈0．05）。结论瘢痕组织形成腰椎术后硬膜外前方瘢痕组织易粘连；瘢痕组织形成晚期硬膜外前方瘢痕组织为不成熟的瘢痕组织。
Objective To analyse the mature degree of epidural anterior and posterior scar tissue after lumbar surgery to prevent and treat the epidural after the lumbar surgery. Methods 24 adult male rats were diveded into 4 groups randomly via 2, 4, 8, 12 weeks. Lamiueetomy was performed in L5-6 in all groups. Labeled streptavidiu biotin was adopted to mark α-SMA and fibrouectiu and desmiu for immuuohistochemical staining, and these results were analysed by computed image processing system and statistical method. Results The result of quantity analysis showed that there was significant difference between the anterior and the posterior on the quantity of the α-SMA and fibroueetiu FN in the epidural scar tissue during 8-12 weeks （P〈0.05）. Conclusion During the later period postoperative lumbar, the epidural fibrosis formed and adhered easily;The epidural scar tissues are in the state of premature during 8-12 weeks.
出处
《中国现代医生》
2007年第12X期18-19,共2页
China Modern Doctor
