摘要
目的 研究胰岛素样生长因子结合蛋白相关蛋白1（insulin-like growth factor binding proteinrelated protein-1，IGFBP-rP1）在转化生长因子β1（tronstorming growth factor β1，TGFβ1）诱导的肝星状细胞中表达的变化及抗IGFBP-rP1抗体对TGFβ1，诱导的肝星状细胞产生Ⅰ型胶原的影响。方法大鼠肝星状细胞株（HSC-T6）体外培养，分别设空白对照组（加入等量PBS）、不同浓度的TGFβ1，处理组、不同浓度的抗IGFBP-rP1抗体处理组，处理因素作用24h，采用免疫组织化学染色、Western blot检测IGFBP-rP1在HSC-T6中的表达，ELISA检测Ⅰ型胶原的含量并进行IGFBP-rP1与Ⅰ型胶原的相关性分析。结果1 TGFβ1，各处理组IGFBP-rP1的表达均较空白对照组显著增强。抗IGFBP-rP1抗体可以拮抗TGFβ1，诱导的HSC-T6产生的过多Ⅰ型胶原。IGFBP-rP1与Ⅰ型胶原呈正相关（r=0．833，P〈0．01）。结论 IGFBP-rP1参与了肝纤维化的形成，且可能在肝纤维化的发生、发展中占有重要地位。
Objective To study the expression of insulin-like growth factor binding protein-related protein-Ⅰ （IGFBP-rP1） in TGFβ1 induced hepatic stellate cell and the effects of anti-ⅠGFBP-rP1 antibody on the collagen Ⅰ production of TGFβ1 induced hepatic stellate cell. Methods Rat hepatic stellate cell line （HSC-T6） was cultured in vitro , and then was incubated with phosphate buffered solution （ blank control group）, TGFβ1 at 2 μg/L or 4 μg/L （TGFβ1 group）, anti-ⅠGFBP-rP1 antibody at 0.1 μg/L, 1 μg/L or 2 μg/L （anti-ⅠGFBP-rP1 antibody group） or both （TGFβ1 ＋ anti-ⅠGFBP-rP1 antibody group）. Twenty-four hours later, the expression of IGFBP-rP1 in HSC-T6 was detected by immunohistochemical staining and western blotting; the content of collagen Ⅰ in culture supernatant of HSC-T6 was measured by ELISA; the relationship between IGFBP-rP1 and collagen I was analyzed. Results The expression of IGFBP-rP1 in TGFβ1 group was significantly higher than that in blank control group. The result of ELISA showed that anti-ⅠGFBP-rP1 antibody antagonized the over-production of collagen I by TGFβ1 induced HSC-T6. There was a positive correlation between IGFBP-rP1 and collagen Ⅰ （ r = 0.833,p 〈0.01 ）. Conclusions IGFBP-rP1 may play an important role in the formation and development of liver fibrosis.
出处
《中华消化外科杂志》
CAS
CSCD
2008年第1期49-52,共4页
Chinese Journal of Digestive Surgery
基金
国家自然科学基金资助项目（30740031）
国家人事部留学回国人员科技活动项目择优资助（200499）
国家教育部留学归国人员科研启动基金（2005383）
山西省自然科学基金项目（20051092）
山西省教育厅青年学术带头人基金（2005017）
