摘要
目的研究紫玉盘茎叶石油醚部位的抗肿瘤活性。方法乙醇回流提取后分别采用石油醚、氯仿、乙酸乙酯和正丁醇萃取有效部位。应用小鼠S180实体瘤模型,研究紫玉盘茎叶石油醚部位的体内抗肿瘤作用。采用MTT检测法检测紫玉盘茎叶石油醚部位的体外抗肿瘤活性。结果紫玉盘茎叶石油醚部位对S180实体瘤呈剂量依赖性抑制作用,600mg/kg组的瘤重抑制百分率达51.04%,与生理盐水组比较差异有显著性(P<0.01)。MTT检测显示,紫玉盘茎叶石油醚20μg/mL对SMMC-7721、SGC-7901和KB细胞的增殖有明显抑制作用,与生理盐水组比较差异有显著性(P<0.01)。结论紫玉盘茎叶石油醚部位具有一定的抗肿瘤作用,其机制有待进一步研究。
[ Objective ] To investigate anticancer effects of petroleum ether fraction of stem and leaves of Uvaria microcarpa. [Methods] Efficient ingredient was leached from alcohol extract of stem and leaves of Uvaria microcarpa by petroleum ether, chloroform, ethyl acetoacetate and n-butyl alcohol respectively. Anticancer effects of petroleum ether fraction were experimented by entity S180 mice model in vivo and MTT reduction test in vitro. [Resuits] Petroleum ether fraction could inhibited entity S180 growth in a dose-dependent manner, the inhibitive percentage of carcinoma weight was 51.04% in 600 mg/kg group and there was obviously differentiation when comparing with normal saline group （P 〈0.01）. Petroleum ether fraction at 20 μg/mL could inhibit cell growth of SGC-7901, SMMC-7721 and KB cell lines when comparing with NS group （P 〈0.01）. [Conclusion] This study confirms that petroleum ether fraction of Uvaria microcarpa has significant anticancer effect; however, the mechanism remains to be studied.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第1期47-50,共4页
China Journal of Modern Medicine
基金
广西自然科学基金(桂科基0342002-3)
关键词
紫玉盘
抗肿瘤
S180
MTT法
Uaria microcarpa
anticancer
S180
MTT reduction test
