摘要
采用室内水培试验,研究不同浓度的外源铜处理对夏腊梅(Sinocalycanthus chinensis)种子萌发、根伸长抑制率、呼吸强度、丙二醛及抗氧化酶系统的影响.结果表明,(1)铜浓度与夏腊梅根伸长抑制率呈显著正相关(P<0.01),但与萌发率相关性不显著.(2)铜处理对夏腊梅种子的过氧化物酶(POD EC1.11.1.7)具有激活效应,且随着铜浓度的增加激活效应逐渐增强.丙二醛(MDA)含量随铜浓度的增大先略降而后逐渐升高.对超氧化物歧化酶(SOD EC 1.15.1.1)和过氧化氢酶(CATEC 1.11.1.6)存在胁迫初期低浓度下的激活效应,随着铜浓度的增加及胁迫时间的延长,表现为抑制效应,且随着铜浓度的增加抑制效应逐渐增强.
Effects of excess copper on seed germination,inhibition of root elongation,respiratory intensity,melondialodehyde（MDA）,and antioxidant enzymes of Sinocalycanthus chinensis were investigated.Results showed that inhibition of root elongation was positively correlated with Cu concentration（P〈0.01）.On the other side,the correlation between inhibition rate of seed germination and Cu concentration was not significant.Copper stimulated peroxidase（POD EC 1.11.1.7） activity and the activity increased gradually with the increase of Cu concentration.MDA content decreased at lower concentration and increased gradually with Cu concentration.In terms of the superoxide dismutase（SOD EC 1.15.1.1） and catalase（CAT EC 1.11.1.6） activities at initial stage of Cu stress,both activities were stimulated at the lower concentration range of Cu.With the extension of Cu stress time and the increase in Cu concentration,the stimulative effects were inhibited and the inhibition increased gradually.
出处
《环境化学》
CAS
CSCD
北大核心
2008年第1期44-48,共5页
Environmental Chemistry
基金
浙江省自然科学基金(Y504220)资助项目
关键词
夏腊梅
铜
种子萌发
抗氧化酶
Sinocalycanthus chinensis,copper,seed germination,antioxidant enzymes.
