摘要
研究结果发现，醋酸汞Ｈｇ２＋（１０－８－１０－５ｍｏｌ／Ｌ）和醋酸铅Ｐｂ２＋（１０－７－１０－４ｍｏｌ／Ｌ）分别使得经ｈＣＧ（１００ｍＩＵ）刺激处理２４小时的Ｌｅｙｄｉｇ细胞内ｃＡＭＰ含量显著下降；同时Ｈｇ２＋（１０－７－１０－５ｍｏｌ／Ｌ）和Ｐｂ２＋（１０－６－１０－４ｍｏｌ／Ｌ）也使得Ｌｅｙｄｉｇ细胞Ｔ含量明显降低并且具有明显的剂量－反应关系。醋酸锌（Ｚｎ２＋）对原代培养的Ｌｅｙｄｉｇ细胞的上述观察指标均无影响，表明用这些指标的变化可以评价Ｈｇ２＋和Ｐｂ２＋对大鼠睾丸Ｌｅｙｄｉｇ的某些毒性。
In this study, the effect of mercury acetate (Hg 2+ ) and lead acetate (Pb 2+ ) on primary cultured Leydig cell's hCG stimulated testosterone(T) concentration and intracellular cAMP concentration were examined The results demonstrated that Hg 2+ (10 -8 —10 -5 mol/L) and Pb 2+ (10 -7 —10 -4 mol/L) resulted in a dose related decrease hCG stimulated intracellular concentration of cAMP and also Hg 2+ (10 -7 —10 -5 mol/L) and Pb 2+ (10 -6 —10 -4 mol/L) caused deduction of hCG stimulated concentration of T respectively.Our research shows that zinc acetate (Zn 2+ ),which is known to be essential for the maintenance of germinal epithelium in the testes,does not result in any varieties of the indices showed above.So we can use these indices as biochemical markers to evaluate the toxic effects of Hg 2+ and Pb 2+ on Leydig cell of the testes.
出处
《卫生毒理学杂志》
CSCD
1997年第2期81-83,共3页
Journal of Health Toxicology
基金
国家自然科学基金
