摘要
基于核苷类药物中间体氰基乙基-5-氯尿嘧啶（CECU）与血清白蛋白相互作用生成复合物，导致血清白蛋白的内源荧光产生特异性变化，而建立了以氰基乙基-5-氯尿嘧啶为荧光探针，用固定波长同步荧光光谱技术测定人血清白蛋白（HSA）和牛血清白蛋白（BSA）的新方法。结果表明，在最佳实验条件下，体系的荧光强度与人血清白蛋白和牛血清白蛋白分别在2．76～497μg／mL和3．90～507μg/mL范围内呈良好的线性关系，检出限分别为1．64和1．72μg/mL（n=11）。该方法灵敏度高、稳定时间长、选择性好、线性范围宽。方法已用于血清样品中蛋白质的快速测定。
Cyanogen-ethylic-5-chlorinuracil uracil （CECU）, a kind of nucleotide as pharmceutic intermediate, can react with serum albumin to form a complex, resulting in a special change of the synchronous fluorescence of serum albumin. Based on this, a new method for the determination of proteins by synchronous fluorescence spectrum was established using CECU as a fluorescence probe. The experiment shows that, under the optimum conditions, the synchronous fluorescence intensity of the system is in proportion to the concentration of proteins in the range of 2.76 - 497 μg/mL for human serum albumin （lISA） and 3.90 - 507 p.g/mL for bovine serum albumin （BSA）. The detection limits were 1.64 and 1.72μg/mL （n = 11 ） for lISA and BSA, respectively. The method is simple, rapid, sensitive, stable, with good selectivity and wide linear range. It had been applied to the determination of proteins in human and bovine serum samples successfully.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第1期23-26,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(20673034)
河南省青年骨干教师(200470)
河南省教育厅自然科学基金(2006150012)
河南省新乡市科技攻关(06G086)项目资助
关键词
氰基乙基-5-氯尿嘧啶
人血清白蛋白
牛血清白蛋白
同步荧光光谱
Cyanogen-ethylic-5-chlorinuracil uracil
Human senun albumin
Bovine senun albumin
Synchronous fluorescence spectrum
